Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc20 Double Nickase Plasmid (h): sc-400583-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc20 Double Nickase Plasmid (h) and Cdc20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC20. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc20 Antibody (E-7): sc-13162
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc20 Double Nickase Plasmid (h) | sc-400583-NIC | 20 µg | $410.00 | |||
Cdc20 Double Nickase Plasmid (h2) | sc-400583-NIC-2 | 20 µg | $410.00 |
CDC20 encodes Cdc20, a core co-activator of the anaphase-promoting complex/cyclosome (APC/C) that coordinates spindle assembly checkpoint signaling with ubiquitin-mediated degradation of key mitotic regulators, including securin and cyclin B1. By promoting timely anaphase onset and mitotic exit, Cdc20 helps maintain chromosome segregation fidelity and overall genomic stability. CDC20 activity is regulated through checkpoint proteins such as MAD2 and BUBR1, linking it to cell-cycle surveillance pathways that constrain aneuploidy. Dysregulated CDC20 expression or checkpoint control is frequently associated with proliferative phenotypes and chromosomal instability observed across multiple cancer research contexts.
Cdc20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.