Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD19 CRISPR/Cas9 KO Plasmid (m): sc-419534
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD19 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD19 Antibody (F-3): sc-373897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD19 CRISPR/Cas9 KO Plasmid (m) | sc-419534 | 20 µg | $397.00 |
Overview
Mouse Cd19 encodes CD19, a B cell–restricted surface co-receptor that amplifies signaling from the B cell receptor complex through recruitment of kinases and adaptor proteins, coordinating downstream PI3K/AKT and MAPK pathway activity. By tuning activation thresholds, calcium flux, proliferation, and survival, CD19 contributes to germinal center responses and effective humoral immunity. Altered CD19-dependent signaling and B cell differentiation states are widely used as mechanistic readouts in immunology and hematologic disease models, including studies of aberrant B cell activation and lineage identity.
CD19 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd19 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD19 protein expression.
This CRISPR knockout system enables efficient generation of Cd19-deficient cell models for investigation of CD19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd19 exon(s) critical for CD19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD19 CRISPR/Cas9 KO Plasmid (m) and CD19 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD19 HDR Plasmid (m) and CD19 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd19 homology arms to support homology-directed repair at defined Cd19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.