C6orf130 CRISPR/Cas9 KO Plasmid (h): sc-415131
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C6orf130 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C6orf130 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C6orf130 CRISPR/Cas9 KO Plasmid (h) | sc-415131 | 20 µg | $397.00 |
Overview
OARD1 (C6orf130) is a human protein-coding gene with limited functional annotation, but available evidence suggests roles in regulating cellular homeostasis through protein interactions that influence gene expression and stress-responsive processes. Expression patterns and genetic associations have implicated this locus in pathways relevant to neuronal function and metabolic regulation, where altered activity may contribute to complex disease phenotypes. As an incompletely characterized factor, C6orf130 is frequently investigated to define its subcellular localization, interaction partners, and downstream transcriptional or signaling consequences. Loss-of-function studies help clarify whether OARD1 impacts cell growth, differentiation, or adaptive responses to proteotoxic or oxidative stress.
C6orf130 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OARD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OARD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OARD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C6orf130 protein expression.
This CRISPR knockout system enables efficient generation of OARD1-deficient cell models for investigation of C6orf130 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OARD1 exon(s) critical for C6orf130 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OARD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C6orf130 CRISPR/Cas9 KO Plasmid (h) and C6orf130 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OARD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C6orf130 HDR Plasmid (h) and C6orf130 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OARD1 homology arms to support homology-directed repair at defined OARD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.