Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
γ-catenin Double Nickase Plasmid (h): sc-401640-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- γ-catenin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- γ-catenin Double Nickase Plasmid (h) and γ-catenin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting JUP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γ-catenin Antibody (A-6): sc-514115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γ-catenin Double Nickase Plasmid (h) | sc-401640-NIC | 20 µg | $410.00 | |||
γ-catenin Double Nickase Plasmid (h2) | sc-401640-NIC-2 | 20 µg | $410.00 |
JUP encodes human γ-catenin (plakoglobin), an armadillo-repeat protein that links cadherin adhesion complexes to the actin and intermediate filament cytoskeleton. At adherens junctions and desmosomes, γ-catenin supports cell–cell adhesion, tissue integrity, and mechanotransduction, and it can also influence Wnt/β-catenin–associated transcriptional programs through shared binding partners. Altered JUP expression or junctional localization is associated with disrupted epithelial barrier function and changes in cell migration and differentiation, making it relevant to studies of cancer biology and cardiocutaneous phenotypes tied to desmosome dysfunction. These roles place γ-catenin at the intersection of junction assembly, cytoskeletal organization, and signaling networks that control tissue homeostasis.
γ-catenin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the JUP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within JUP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt JUP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of JUP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.