Trichohyalin Antibody (AE15): sc-80607

Trichohyalin Antibody (AE15) is a mouse monoclonal IgG1 kappa light chain antibody that detects Trichohyalin of mouse, rat, and human origin by immunofluorescence (IF) and flow cytometry (FCM). Anti-Trichohyalin antibody (AE15) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Trichohyalin is a crucial structural protein predominantly found in the medulla and inner root sheath of hair follicles, where Trichohyalin plays a vital role in the formation and organization of the cornified cell envelope. This structural protein is characterized by a nine-domain structure, which includes two EF-hand calcium-binding motifs essential for cellular processes. As a member of the S100-fused protein family, Trichohyalin is involved in regulating keratin intermediate filaments and interacts with peripheral cell envelope barrier proteins, contributing to hair follicle structure integrity and resilience. Trichohyalin′s ability to undergo post-translational modifications, such as cross-linking by transglutaminase and deamination by peptidylarginine deaminase, enhances functional diversity and importance in skin and hair biology.

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Trichohyalin Antibody (AE15) References:

1. Expression of trichohyalin in dermatological disorders: a comparative study with involucrin and filaggrin by immunohistochemical staining.  |  Lee, SC., et al. 1999. Acta Derm Venereol. 79: 122-6. PMID: 10228630
2. Expression of trichohyalin in skin disorders.  |  Manabe, M. and Ogawa, H. 1999. Exp Dermatol. 8: 312. PMID: 10439242
3. Trichohyalin mechanically strengthens the hair follicle: multiple cross-bridging roles in the inner root shealth.  |  Steinert, PM., et al. 2003. J Biol Chem. 278: 41409-19. PMID: 12853460
4. Trichohyalin: presence in the granular layer and stratum corneum of normal human epidermis.  |  Hamilton, EH., et al. 1991. J Invest Dermatol. 96: 666-72. PMID: 1708794
5. Interaction of trichohyalin with intermediate filaments: three immunologically defined stages of trichohyalin maturation.  |  O'Guin, WM., et al. 1992. J Invest Dermatol. 98: 24-32. PMID: 1728637
6. Trichohyalin is a potential major autoantigen in human alopecia areata.  |  Leung, MC., et al. 2010. J Proteome Res. 9: 5153-63. PMID: 20722389
7. Trichohyalin-like proteins have evolutionarily conserved roles in the morphogenesis of skin appendages.  |  Mlitz, V., et al. 2014. J Invest Dermatol. 134: 2685-2692. PMID: 24780931
8. Existence of trichohyalin-keratohyalin hybrid granules: co-localization of two major intermediate filament-associated proteins in non-follicular epithelia.  |  Manabe, M. and O'Guin, WM. 1994. Differentiation. 58: 65-75. PMID: 7532602
9. The structure of human trichohyalin. Potential multiple roles as a functional EF-hand-like calcium-binding protein, a cornified cell envelope precursor, and an intermediate filament-associated (cross-linking) protein.  |  Lee, SC., et al. 1993. J Biol Chem. 268: 12164-76. PMID: 7685034
10. Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation.  |  Ishida-Yamamoto, A., et al. 1997. Br J Dermatol. 137: 9-16. PMID: 9274619
11. The fate of trichohyalin. Sequential post-translational modifications by peptidyl-arginine deiminase and transglutaminases.  |  Tarcsa, E., et al. 1997. J Biol Chem. 272: 27893-901. PMID: 9346937
12. Biochemical evidence that small proline-rich proteins and trichohyalin function in epithelia by modulation of the biomechanical properties of their cornified cell envelopes.  |  Steinert, PM., et al. 1998. J Biol Chem. 273: 11758-69. PMID: 9565599

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Trichohyalin Antibody (AE15) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Trichohyalin Antibody (AE15) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Trichohyalin Antibody (AE15) Specifications

Alternate Product Name: Trichohyalin

Product Clone Name: AE15

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Trichohyalin

Epitope Species: human

Product Reactivity/Detection: Trichohyalin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Trichohyalin Antibody (AE15): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Trichohyalin antibody is Trichohyalin Antibody (E-11): sc-376684.