Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tom20 Double Nickase Plasmid (h): sc-400041-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tom20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tom20 Double Nickase Plasmid (h) and Tom20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TOMM20. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tom20 Antibody (F-10): sc-17764
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tom20 Double Nickase Plasmid (h) | sc-400041-NIC | 20 µg | $410.00 | |||
Tom20 Double Nickase Plasmid (h2) | sc-400041-NIC-2 | 20 µg | $410.00 |
TOMM20 encodes Tom20, a core receptor of the translocase of the outer mitochondrial membrane (TOM) complex that recognizes N-terminal mitochondrial targeting sequences and initiates import of nuclear-encoded proteins into mitochondria. By coordinating with other TOM components and downstream TIM translocases, Tom20 supports mitochondrial biogenesis, respiratory chain assembly, and proteostasis that influence oxidative phosphorylation, redox homeostasis, and apoptosis signaling. Perturbation of mitochondrial protein import can promote bioenergetic stress, altered mitochondrial dynamics, and innate immune activation linked to neurodegeneration, cardiometabolic dysfunction, and tumor-associated metabolic remodeling. TOMM20 is therefore a useful node for studying mitochondrial quality control pathways and stress-responsive signaling networks.
Tom20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TOMM20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TOMM20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TOMM20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TOMM20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.