T2R38 Double Nickase Plasmid (h): sc-402279-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T2R38 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- T2R38 Double Nickase Plasmid (h) and T2R38 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TAS2R38. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T2R38 Double Nickase Plasmid (h) | sc-402279-NIC | 20 µg | $410.00 | |||
T2R38 Double Nickase Plasmid (h2) | sc-402279-NIC-2 | 20 µg | $410.00 |
Human TAS2R38 encodes the bitter taste receptor T2R38, a class A GPCR best known for sensing thiourea-containing compounds and initiating canonical GPCR signaling through heterotrimeric G proteins, second-messenger modulation, and downstream MAPK-responsive transcriptional programs. Beyond gustation, T2R38 expression in airway and other epithelial contexts has been linked to chemosensory regulation of innate barrier responses, including pathways that influence ciliary activity and antimicrobial signaling. Common TAS2R38 haplotypes alter receptor responsiveness and have been studied in relation to interindividual differences in bitter perception, dietary behavior, and host–environment interactions. These features make TAS2R38 a useful model for dissecting GPCR ligand specificity, signal transduction dynamics, and genotype-dependent phenotypes.
T2R38 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TAS2R38 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TAS2R38. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TAS2R38 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TAS2R38-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.