Date published: 2026-7-11

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Six4 Antibody (34-M): sc-81984

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Datasheets
  • Six4 Antibody (34-M) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 100 µg/ml
  • raised against recombinant Six4 of human origin
  • recommended for detection of Six4 of human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Six4 Antibody (34-M). This work is in progress.
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Six4 Antibody (34-M) is a mouse monoclonal IgG1 antibody that detects Six4 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Six4, also known as AREC3, is a 760 amino acid nuclear protein that plays a crucial role in myogenesis and neurogenesis, as well as in the development of various organs. Six4 is part of the Six/Sine oculis homeobox family, characterized by a conserved DNA-binding domain that allows Six4 to regulate gene expression during development. The presence of a single Six domain in Six4, which functions as a homeobox DNA-binding motif, is essential for interaction with specific DNA sequences, thereby influencing the transcription of target genes. Notably, Six4 shares 90% sequence similarity with its mouse counterpart, indicating that both proteins likely exhibit similar DNA-binding properties and functional roles in developmental processes. The ability of Six4 to modulate gene expression is vital for proper cellular differentiation and organogenesis, making Six4 an important focus of study in developmental biology and related fields.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Six4 Antibody (34-M)

sc-81984
100 µg/ml
$339.00

I am using Six4 (34-M): sc-81984 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Strong band in WB in HeLa nuclear extractStrong band in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-07-16
Rated 3 out of 5 by from Produced good immunofluorescence nuclearProduced good immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-15
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Six4 Antibody (34-M) is rated 3.0 out of 5 by 2.
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