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Rhotekin Antibody (AA-18): sc-100426

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Datasheets
  • Rhotekin Antibody (AA-18) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 100 µg/ml
  • raised against recombinant Rhotekin of human origin
  • recommended for detection of Rhotekin of human origin by WB, IP, IF, IHC(P) and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rhotekin Antibody (AA-18). This work is in progress.
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Rhotekin Antibody (AA-18) is a mouse monoclonal IgG1 antibody that detects Rhotekin in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Rhotekin plays a crucial role in regulating the actin cytoskeleton, particularly in forming and maintaining stress fibers and focal adhesions, which are essential for cellular shape, motility, and division. Rhotekin acts as a downstream effector of the Rho GTPase signaling pathway, linking Rho to various cellular processes such as cytokinesis and cell adhesion. Rhotekin′s ability to interact with other proteins, including Rho and the serine/threonine kinases Rock-1 and Rock-2, highlights its importance in mediating Rho signaling effects. Rhotekin′s structural characteristics, which include a Rho-binding domain and a coiled-coil region, are vital for binding to Rho and facilitating downstream signaling events. Anti-Rhotekin antibody (AA-18) helps researchers gain insights into cytoskeleton dynamics and regulatory mechanisms governing cell behavior, making this tool valuable for studies in cell biology and related fields.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rhotekin Antibody (AA-18)

sc-100426
100 µg/ml
$339.00

Can sc-100426: Rhotekin (AA-18) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, sc-100426: Rhotekin (AA-18) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust immunoperoxidase cytoplasmic stainingRobust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-02-26
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Rhotekin Antibody (AA-18) is rated 4.0 out of 5 by 2.
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