RBM47 Double Nickase Plasmid (m): sc-434185-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM47 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RBM47 Double Nickase Plasmid (m) and RBM47 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Rbm47. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM47 Double Nickase Plasmid (m) | sc-434185-NIC | 20 µg | $410.00 | |||
RBM47 Double Nickase Plasmid (m2) | sc-434185-NIC-2 | 20 µg | $410.00 |
Rbm47 encodes the RNA-binding motif protein RBM47, a conserved regulator of post-transcriptional gene expression that binds specific transcripts to influence mRNA stability, localization, and alternative splicing. In mouse cells, RBM47 contributes to RNA processing programs that shape epithelial differentiation and stress-responsive transcriptional outputs, interfacing with broader RNA metabolism pathways that include spliceosome-associated factors and RNA surveillance. Altered RBM47 activity has been associated with dysregulated gene expression networks relevant to development and epithelial–mesenchymal plasticity, making it a useful node for studying mechanisms that link RNA regulation to cell-state transitions. These features position RBM47 as a relevant target for dissecting how RNA-binding proteins coordinate transcriptome remodeling in normal physiology and disease-relevant contexts.
RBM47 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Rbm47 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Rbm47. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Rbm47 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Rbm47-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.