Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OTUD7B Double Nickase Plasmid (h): sc-403268-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUD7B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OTUD7B Double Nickase Plasmid (h) and OTUD7B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OTUD7B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OTUD7B Antibody (H-4): sc-514402
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUD7B Double Nickase Plasmid (h) | sc-403268-NIC | 20 µg | $410.00 | |||
OTUD7B Double Nickase Plasmid (h2) | sc-403268-NIC-2 | 20 µg | $410.00 |
OTUD7B (also known as Cezanne) encodes an OTU-family deubiquitinase that edits Lys11- and Lys63-linked ubiquitin chains to shape ubiquitin-dependent signaling and protein stability. By counteracting E3 ligase–driven ubiquitination, OTUD7B influences proteostasis and inflammatory signal transduction, including NF-κB pathway dynamics and related innate immune responses. OTUD7B activity has been connected to regulation of cytokine signaling networks and cellular stress adaptation, making it relevant to studies of immune dysregulation, tumor-associated inflammation, and ubiquitin pathway vulnerabilities. Functional perturbation of OTUD7B provides a route to dissect deubiquitination-dependent control points in transcriptional programs and receptor-proximal signaling complexes.
OTUD7B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OTUD7B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OTUD7B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OTUD7B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OTUD7B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.