KOR-3 CRISPR/Cas9 KO Plasmid (h): sc-402526
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KOR-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KOR-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KOR-3 CRISPR/Cas9 KO Plasmid (h) | sc-402526 | 20 µg | $397.00 |
Overview
OPRL1 encodes the nociceptin/orphanin FQ receptor (also termed KOR-3), a class A GPCR that couples primarily to Gi/Go to suppress adenylyl cyclase activity, reduce cAMP signaling, and modulate ion channel conductance. Receptor activation regulates synaptic transmission and neuronal excitability, influencing neuropeptide-mediated control of pain processing, stress responsivity, reward circuitry, and autonomic functions. Downstream signaling intersects with MAPK/ERK pathways, β-arrestin–dependent trafficking, and receptor desensitization/internalization processes that shape cellular responses over time. Altered OPRL1/KOR-3 signaling has been associated with neuropsychiatric and neurobehavioral phenotypes, making it relevant for mechanistic studies of neural network regulation and GPCR signaling dynamics.
KOR-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OPRL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OPRL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OPRL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KOR-3 protein expression.
This CRISPR knockout system enables efficient generation of OPRL1-deficient cell models for investigation of KOR-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OPRL1 exon(s) critical for KOR-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OPRL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KOR-3 CRISPR/Cas9 KO Plasmid (h) and KOR-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OPRL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KOR-3 HDR Plasmid (h) and KOR-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OPRL1 homology arms to support homology-directed repair at defined OPRL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.