H+/K+ ATPase β Double Nickase Plasmid (h): sc-403057-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- H+/K+ ATPase β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- H+/K+ ATPase β Double Nickase Plasmid (h) and H+/K+ ATPase β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP4B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: H+/K+ ATPase β Antibody (C-4): sc-374094
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H+/K+ ATPase β Double Nickase Plasmid (h) | sc-403057-NIC | 20 µg | $410.00 | |||
H+/K+ ATPase β Double Nickase Plasmid (h2) | sc-403057-NIC-2 | 20 µg | $410.00 |
ATP4B encodes the β subunit of the gastric H+/K+ ATPase, a heterodimeric P-type ATPase that drives proton secretion in exchange for potassium across the apical membrane of parietal cells. The β subunit supports catalytic stability and membrane trafficking of the α subunit, enabling sustained acidification required for digestive physiology and epithelial homeostasis. This complex participates in ion transport and pH regulation pathways that influence mucosal barrier function and signaling in the gastric epithelium. Altered ATP4B expression and disrupted gastric acid secretory programs have been associated with gastric mucosal pathology and are frequently investigated in the context of inflammation-associated remodeling and gastric cancer biology.
H+/K+ ATPase β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP4B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP4B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP4B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP4B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.