HNF-3β Double Nickase Plasmid (h): sc-400210-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HNF-3β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HNF-3β Double Nickase Plasmid (h) and HNF-3β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HNF-3β Antibody (H-4): sc-374376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HNF-3β Double Nickase Plasmid (h) | sc-400210-NIC | 20 µg | $410.00 | |||
HNF-3β Double Nickase Plasmid (h2) | sc-400210-NIC-2 | 20 µg | $410.00 |
FOXA2 encodes hepatocyte nuclear factor 3-beta (HNF-3β), a forkhead box transcription factor that acts as a pioneer factor to open chromatin and establish lineage-specific transcriptional programs. In human development and adult tissue homeostasis, FOXA2 regulates endoderm patterning and differentiation, with prominent roles in liver, pancreas, lung, and gastrointestinal epithelia. Its activity integrates with networks involving WNT, TGF-β, and Notch signaling, coordinating metabolic gene expression, epithelial identity, and organ-specific maturation. Dysregulation of FOXA2-linked transcriptional circuits has been associated with altered differentiation states and disease-relevant phenotypes in metabolic disorders and cancer biology.
HNF-3β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.