GRASP65 Antibody (H-2): sc-365434

GRASP65 Antibody (H-2) is a mouse monoclonal IgG1 antibody that detects GRASP65 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRASP65, also known as Golgi reassembly stacking protein 65, plays a crucial role in the organization and maintenance of the Golgi apparatus, which is essential for proper protein processing and trafficking within the cell. GRASP65 is primarily located in the cis-Golgi and is vital for the stacking of Golgi cisternae, working in conjunction with its homolog, GRASP55. The interaction between GRASP65 and other Golgi-associated proteins, such as golgin 45, is significant for the formation of a functional Golgi structure, which is necessary for the efficient transport of proteins from the endoplasmic reticulum to the cell surface. Additionally, GRASP65 undergoes post-translational modifications, including phosphorylation by ERK2, particularly during mitosis, which suggests that GRASP65 activity is regulated in response to cellular signaling pathways. The ability of GRASP65 to interact with various proteins and involvement in the dynamic organization of the Golgi apparatus underscore GRASP65′s importance in maintaining cellular homeostasis and facilitating proper cellular function.

The preferred GRASP65 antibody is GRASP65 Antibody (D-12): sc-374423.