Dynein IC1, axonemal CRISPR/Cas9 KO Plasmid (h): sc-411947
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynein IC1, axonemal CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dynein IC1, axonemal genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynein IC1, axonemal CRISPR/Cas9 KO Plasmid (h) | sc-411947 | 20 µg | $397.00 |
Overview
DNAI1 encodes dynein intermediate chain 1, an axonemal dynein component required for assembly and force transmission within outer dynein arms that power motile cilia and flagella beating. By coupling ATP-driven dynein motor activity to microtubule doublets, Dynein IC1, axonemal contributes to coordinated ciliary waveform generation and mucociliary clearance processes. DNAI1 function intersects with pathways governing cilia biogenesis, axonemal organization, and intraflagellar transport-dependent maintenance of motile cilia structure. Disruption of DNAI1 is associated with primary ciliary dyskinesia, linking altered axonemal dynein integrity to impaired ciliary motility and downstream developmental and respiratory phenotypes.
Dynein IC1, axonemal CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAI1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAI1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAI1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dynein IC1, axonemal protein expression.
This CRISPR knockout system enables efficient generation of DNAI1-deficient cell models for investigation of Dynein IC1, axonemal signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAI1 exon(s) critical for Dynein IC1, axonemal function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAI1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dynein IC1, axonemal CRISPR/Cas9 KO Plasmid (h) and Dynein IC1, axonemal CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAI1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dynein IC1, axonemal HDR Plasmid (h) and Dynein IC1, axonemal HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAI1 homology arms to support homology-directed repair at defined DNAI1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.