dsg3 Double Nickase Plasmid (h): sc-401809-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- dsg3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- dsg3 Double Nickase Plasmid (h) and dsg3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DSG3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: dsg3 Antibody (5H10): sc-23912
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dsg3 Double Nickase Plasmid (h) | sc-401809-NIC | 20 µg | $410.00 | |||
dsg3 Double Nickase Plasmid (h2) | sc-401809-NIC-2 | 20 µg | $410.00 |
Desmoglein 3 (DSG3; dsg3) is a calcium-dependent desmosomal cadherin that mediates strong cell–cell adhesion in stratified epithelia through trans-interactions and linkage to plakoglobin, plakophilins, and desmoplakin, thereby coupling adhesion complexes to intermediate filaments. By stabilizing desmosome assembly and remodeling, DSG3 contributes to epithelial barrier integrity, tissue morphogenesis, and mechanical stress responses, with downstream effects on cytoskeletal organization and contact-regulated signaling. Perturbation of DSG3-dependent adhesion is associated with loss of epithelial cohesion and altered differentiation programs. DSG3 is also widely used as a marker of epithelial lineage and desmosome biology in studies of skin and mucosal tissue homeostasis.
dsg3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DSG3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DSG3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DSG3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DSG3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.