CLC-5 Double Nickase Plasmid (h): sc-404859-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLC-5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLC-5 Double Nickase Plasmid (h) and CLC-5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLCN5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLC-5 Double Nickase Plasmid (h) | sc-404859-NIC | 20 µg | $410.00 | |||
CLC-5 Double Nickase Plasmid (h2) | sc-404859-NIC-2 | 20 µg | $410.00 |
Human CLCN5 encodes CLC-5, an endosomal voltage-gated Cl⁻/H⁺ exchanger that supports vesicular acidification and chloride conductance required for receptor-mediated endocytosis and membrane protein trafficking. CLC-5 localizes predominantly to early endosomes in polarized epithelial cells, where it functionally couples to V-ATPase activity to regulate luminal pH, cargo sorting, and recycling. Disruption of CLCN5 perturbs proximal tubular reabsorption pathways and endolysosomal homeostasis, and is associated with inherited renal tubular dysfunction such as Dent disease. As a result, CLC-5 is frequently studied in epithelial transport biology, endocytic pathway regulation, and mechanisms linking endosomal ion handling to protein uptake and degradation.
CLC-5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLCN5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLCN5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLCN5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLCN5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.