Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ChM-1 CRISPR/Cas9 KO Plasmid (h): sc-403523
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ChM-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ChM-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ChM-1 Antibody (H-10): sc-365693
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ChM-1 CRISPR/Cas9 KO Plasmid (h) | sc-403523 | 20 µg | $397.00 |
Overview
CNMD encodes chondromodulin-1 (ChM-1), a cartilage-derived secreted glycoprotein implicated in maintaining the avascular microenvironment of cartilage by modulating endothelial cell behavior and extracellular matrix homeostasis. ChM-1 is linked to chondrogenic differentiation programs and tissue remodeling processes that coordinate matrix deposition, cell adhesion, and local growth factor signaling in musculoskeletal tissues. Altered CNMD expression has been reported in contexts of cartilage degeneration and repair, supporting its use as a molecular entry point for studying osteoarticular biology. In addition, CNMD/ChM-1 activity is relevant to investigations of angiogenesis suppression and matrix-regulated signaling in development and disease-associated tissue remodeling.
ChM-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CNMD gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CNMD together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CNMD open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ChM-1 protein expression.
This CRISPR knockout system enables efficient generation of CNMD-deficient cell models for investigation of ChM-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CNMD exon(s) critical for ChM-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CNMD genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ChM-1 CRISPR/Cas9 KO Plasmid (h) and ChM-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CNMD locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ChM-1 HDR Plasmid (h) and ChM-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CNMD homology arms to support homology-directed repair at defined CNMD target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.