CD67 Double Nickase Plasmid (h): sc-405743-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD67 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD67 Double Nickase Plasmid (h) and CD67 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CEACAM8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD67 Antibody (GM2H6): sc-101383
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD67 Double Nickase Plasmid (h) | sc-405743-NIC | 20 µg | $410.00 | |||
CD67 Double Nickase Plasmid (h2) | sc-405743-NIC-2 | 20 µg | $410.00 |
CEACAM8 encodes CD67, a granulocyte-associated member of the carcinoembryonic antigen-related cell adhesion molecule family that is enriched on human neutrophils and upregulated during activation. CD67 participates in cell–cell interactions and contributes to neutrophil adhesion, degranulation-associated responses, and modulation of inflammatory signaling in the innate immune system. Through its role in leukocyte trafficking and effector function, CEACAM8 is frequently studied in pathways linked to mucosal immunity, antimicrobial defense, and cytokine-driven inflammation. Altered expression patterns in myeloid cells and inflamed tissues have made CEACAM8 a useful research marker in studies of inflammatory disorders and tumor-associated immune infiltration.
CD67 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CEACAM8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CEACAM8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CEACAM8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CEACAM8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.