Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Calgranulin B CRISPR/Cas9 KO Plasmid (h): sc-400801
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calgranulin B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Calgranulin B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calgranulin B Antibody (B-5): sc-376772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calgranulin B CRISPR/Cas9 KO Plasmid (h) | sc-400801 | 20 µg | $397.00 |
Overview
S100A9 encodes calgranulin B, a calcium-binding S100 family protein that forms heterodimers with S100A8 and is abundantly expressed in neutrophils and inflammatory monocytes. Calgranulin B regulates innate immune signaling and leukocyte trafficking through interactions with pattern-recognition receptors such as TLR4 and RAGE, amplifying NF-κB/MAPK-driven cytokine and chemokine programs. It contributes to antimicrobial responses, oxidative stress regulation, and myeloid cell activation, and is frequently studied as a marker and mediator of acute and chronic inflammation. Dysregulated S100A9 expression has been linked to inflammatory disorders and cancer-associated myeloid programs, including recruitment and functional polarization of suppressive myeloid populations in the tumor microenvironment.
Calgranulin B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the S100A9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the S100A9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the S100A9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Calgranulin B protein expression.
This CRISPR knockout system enables efficient generation of S100A9-deficient cell models for investigation of Calgranulin B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting S100A9 exon(s) critical for Calgranulin B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple S100A9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Calgranulin B CRISPR/Cas9 KO Plasmid (h) and Calgranulin B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the S100A9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Calgranulin B HDR Plasmid (h) and Calgranulin B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by S100A9 homology arms to support homology-directed repair at defined S100A9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.