ADH4 Antibody (NB-B2): sc-134249

ADH4 Antibody (NB-B2) is a mouse monoclonal IgG2a antibody that detects ADH4 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ADH4, or alcohol dehydrogenase 4, is a 380 amino acid protein that plays a crucial role in the metabolism of diverse substrates, such as retinol, hydroxysteroids, ethanol, aliphatic alcohols, and lipid peroxidation products. ADH4 is primarily localized in the cytoplasm and functions as a homodimer, utilizing zinc as a cofactor to facilitate the NAD+-dependent conversion of alcohols to aldehydes or ketones, which is essential for metabolic degradation of alcohols in the body. ADH4′s importance extends beyond enzymatic activity; various post-translational modifications can influence stability, activity, and interactions with other proteins. These modifications can affect ADH4′s function and role in metabolic pathways, highlighting the significance of understanding regulatory mechanisms in both health and disease. Additionally, multiple isoforms of ADH4 arise from alternative splicing, contributing to the complexity of regulation and function. The gene encoding ADH4 is located on human chromosome 4, which contains a cluster of alcohol dehydrogenase genes and represents nearly 6% of the human genome, including some of the largest gene deserts found in human chromosomes. ADH4 Antibody (NB-B2) serves as an invaluable tool for researchers investigating metabolic pathways involving alcohols and their implications in various physiological and pathological conditions.

The preferred ADH4 antibody is ADH4 Antibody (F-6): sc-515217.