ACE Antibody (3H533): sc-69991

ACE Antibody (3H533) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target angiotensin-converting enzyme (ACE), also known as CD143 or Angiotensin Converting Enzyme 1. Anti-ACE antibody (3H533) is raised against human kidney-derived ACE and proficiently detects ACE proteins from mouse, rat, and human origins through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). ACE monoclonal antibody (3H533) is available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, offering versatile options for diverse research needs. Structurally, ACE is a zinc metallopeptidase composed of two homologous domains, each containing an active site essential for enzymatic activity in the renin-angiotensin system. This dimeric structure is crucial for converting Angiotensin I to the potent vasopressor hormone Angiotensin II, thereby regulating blood pressure and electrolyte balance. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, contributing to fluid and electrolyte homeostasis. Two primary isoforms of ACE exist: pulmonary ACE (ACEP), a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney, and spleen, and testicular ACE (ACET), expressed exclusively in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Understanding ACE′s intricate structure is essential for elucidating cardiovascular health and disease, making anti-ACE antibody (3H533) an indispensable tool for investigating hypertension, heart failure, and related physiological processes.

The preferred ACE antibody is ACE Antibody (2E2): sc-23908.