UEV3 Activators
UEV3 include a variety of compounds that influence cellular signaling pathways leading to its phosphorylation and subsequent functional activation. Phorbol 12-myristate 13-acetate (PMA) and 12-O-Tetradecanoylphorbol-13-acetate (TPA), both being diesters of phorbol, are known to activate protein kinase C (PKC). Once activated, PKC directly phosphorylates UEV3, which alters its interaction with other proteins and its activity within the cell. Forskolin, by activating adenylate cyclase, raises the levels of cAMP within the cell, which then activates protein kinase A (PKA). PKA, in turn, phosphorylates UEV3, leading to changes in its conformation and function. Similarly, Dibutyryl-cAMP, a cAMP analog, activates PKA, which phosphorylates UEV3, thereby modulating its function in ubiquitination processes.
Ionomycin, by increasing intracellular calcium levels, activates calmodulin-dependent kinase (CaMK), which then can phosphorylate UEV3. The activation of the EGF receptor by Epidermal Growth Factor (EGF) initiates a cascade involving the MAPK/ERK pathway, which can lead to UEV3 phosphorylation. Insulin triggers the PI3K/Akt signaling pathway, another potential route for UEV3 phosphorylation. Inhibitors of protein phosphatases like Okadaic Acid, Calyculin A, Sodium Orthovanadate, and Cantharidin prevent the dephosphorylation of proteins, which maintains UEV3 in a phosphorylated state, thereby sustaining its activity. Anisomycin, which activates stress-activated protein kinases (SAPKs), also plays a role in the phosphorylation and activation of UEV3, particularly under stress conditions. These chemical agents, through their diverse mechanisms, converge on the common endpoint of UEV3 phosphorylation, which is a critical determinant of its functional state within the cell.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC) which can phosphorylate UEV3, leading to its functional activation as it may alter its interaction with other proteins and its activity within the cell. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, increasing cAMP levels, which in turn activates PKA. PKA can phosphorylate UEV3, which might result in its functional activation through conformational changes that affect its activity. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calmodulin-dependent kinase (CaMK). CaMK can then phosphorylate UEV3, leading to its functional activation through enhanced protein interactions or activity. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $153.00 $1224.00 $12239.00 | 82 | |
Insulin receptor activation stimulates the PI3K/Akt signaling pathway, which may lead to the phosphorylation and activation of UEV3, potentially altering its ubiquitin-conjugating activity. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cAMP analog that activates PKA. Activated PKA could phosphorylate UEV3, leading to its functional activation within its role in ubiquitination processes. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid inhibits protein phosphatases PP1 and PP2A, preventing dephosphorylation of proteins, which could result in the sustained activation of UEV3 if it is phosphorylated by upstream kinases. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Similar to Okadaic Acid, Calyculin A inhibits protein phosphatases leading to increased phosphorylation levels of proteins. This could keep UEV3 in an activated state if it is regulated by phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases (SAPKs), which might phosphorylate and thereby activate UEV3 as part of the cellular stress response. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $45.00 $56.00 $183.00 | 142 | |
Sodium Orthovanadate is a phosphatase inhibitor that can prevent dephosphorylation of proteins, potentially resulting in the activation of UEV3 if it undergoes phosphorylation by upstream kinases. | ||||||
Cantharidin | 56-25-7 | sc-201321 sc-201321A | 25 mg 100 mg | $81.00 $260.00 | 6 | |
Cantharidin inhibits serine/threonine protein phosphatases, which could result in the hyperphosphorylation and subsequent activation of UEV3, affecting its ubiquitin-conjugating function. | ||||||