Tensin3 Activators
Chemical activators of Tensin3 can influence its role in cytoskeletal organization and focal adhesion dynamics through various signaling pathways. Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C (PKC), a family of enzymes that are pivotal in modulating the function of proteins like Tensin3 through phosphorylation. This phosphorylation can enhance Tensin3's interaction with other focal adhesion proteins, thereby influencing actin dynamics and cellular adhesion. Similarly, Calyculin A and Okadaic Acid, both inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), prevent the dephosphorylation of proteins, leading to sustained Tensin3 activation. By maintaining Tensin3 in a phosphorylated state, these chemicals ensure its continuous participation in the maintenance and regulation of focal adhesions and the actin cytoskeleton.
Growth factors such as Epidermal Growth Factor (EGF) and Platelet-derived growth factor (PDGF) engage with their respective tyrosine kinase receptors to initiate downstream signaling cascades, particularly the PI3K/Akt and MAPK/ERK pathways. These pathways are known to modulate the activity of numerous proteins involved in cell migration, adhesion, and survival, among them Tensin3. EGF can thus promote the activation of Tensin3, enabling it to fulfill its role in cell motility and adhesion. In the context of extracellular signaling molecules, Lysophosphatidic acid (LPA) acts through G protein-coupled receptors to activate the Rho/ROCK pathway, contributing to Tensin3's role in focal adhesion dynamics. On the other hand, engagement of Fibronectin with integrin receptors initiates signaling that leads to the phosphorylation of focal adhesion components, including Tensin3, thereby potentiating its ability to link the extracellular matrix to the cytoskeleton. Lastly, environmental stimuli such as Hydrogen Peroxide can induce oxidative stress response signaling pathways, leading to the activation of kinases that phosphorylate and regulate Tensin3, integrating it into cellular stress response mechanisms.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is known to phosphorylate a wide range of target proteins. Since Tensin3 is involved in focal adhesion and actin cytoskeleton reorganization, PKC-mediated phosphorylation can lead to Tensin3 functional activation by enhancing its interaction with other focal adhesion proteins and influencing actin dynamics. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A). Inhibition of these phosphatases can result in increased phosphorylation levels of proteins, including Tensin3. By preventing the dephosphorylation, Calyculin A can lead to sustained activation of Tensin3, promoting its role in cytoskeletal organization. | ||||||
Lysophosphatidic Acid | 325465-93-8 | sc-201053 sc-201053A | 5 mg 25 mg | $98.00 $341.00 | 50 | |
LPA acts through G protein-coupled receptors (GPCRs) and can activate the Rho/ROCK pathway, which is crucial for actin cytoskeleton organization and focal adhesion dynamics. As Tensin3 is implicated in focal adhesion assembly, LPA can activate Tensin3 by promoting its association with focal adhesion complexes and influencing cytoskeletal rearrangements. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin binds to its receptor, triggering receptor phosphorylation and the subsequent activation of the PI3K/Akt signaling pathway. Akt can phosphorylate multiple substrates involved in cell survival and growth, and this pathway can facilitate the activation of Tensin3 by promoting its interaction with other proteins in focal adhesion complexes. | ||||||
Fibronectin | sc-29011 sc-29011A | 1 mg 5 mg | $143.00 $504.00 | 94 | ||
Fibronectin engagement with integrin receptors activates focal adhesion kinase (FAK) and Src family kinases, which are involved in the phosphorylation and regulation of focal adhesion components. Through these kinases, Tensin3 can be activated to carry out its role in linking the extracellular matrix to the actin cytoskeleton. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinase (CaMK) and other calcium-sensitive signaling pathways that could lead to the activation of Tensin3 by promoting its participation in cytoskeletal rearrangements and focal adhesion turnover. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
Hydrogen peroxide serves as a signaling molecule that can lead to the activation of various kinases through the oxidative stress response. These kinases can phosphorylate and activate Tensin3, linking it to the cellular response to oxidative stress and possibly enhancing its role in the organization of focal adhesions and the actin cytoskeleton. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is an inhibitor of protein phosphatases PP1 and PP2A, like Calyculin A. By inhibiting these phosphatases, it can lead to increased phosphorylation of Tensin3, thereby promoting its activation and function in focal adhesion and cytoskeletal organization. | ||||||
Thrombin from human plasma | 9002-04-4 | sc-471713 | 100 U | $235.00 | ||
Thrombin is a serine protease that can activate protease-activated receptors (PARs) leading to GPCR signaling. This signaling can consequently activate the Rho/ROCK pathway, which is involved in cytoskeletal dynamics. Tensin3, being a part of the focal adhesion complex, can be activated by the changes induced. | ||||||