SRRM5 Activators
Chemical activators of SRRM5 employ various mechanisms to influence the phosphorylation state and activity of this protein. Calcium Ionophore A23187 and Ionomycin function by increasing the intracellular concentration of calcium ions, a key secondary messenger in cellular signaling. The elevated calcium levels can activate calcium-dependent protein kinases, which are known to phosphorylate SRRM5, thereby modulating its activity. Similarly, Thapsigargin, by inhibiting the SERCA pump, leads to a rise in cytosolic calcium levels, again resulting in the activation of kinases that target SRRM5. In contrast, Forskolin and Dibutyryl cAMP work by elevating intracellular cAMP, which in turn activates protein kinase A (PKA). PKA directly phosphorylates SRRM5, leading to changes in its conformation and function.
Compounds such as Phorbol 12-myristate 13-acetate (PMA) and 4-Phorbol 12,13-didecanoate activate protein kinase C (PKC). This kinase phosphorylates serine and threonine residues on SRRM5, influencing its activation state. Inhibition of protein phosphatases plays a crucial role as well; Okadaic Acid and Calyculin A inhibit protein phosphatases 1 and 2A, which normally dephosphorylate proteins. The inhibition of these phosphatases prevents the dephosphorylation of SRRM5, sustaining its phosphorylated, active state. Anisomycin activates stress-activated protein kinases, which may phosphorylate and activate SRRM5. Lastly, Cyclosporin A's inhibition of calcineurin indirectly maintains the phosphorylation of SRRM5, by preventing the dephosphorylation of proteins in the calcineurin pathway. Each of these chemicals, through their unique modes of action, converge on the common endpoint of modulating the phosphorylation status and activity of SRRM5.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
Calcium Ionophore A23187 specifically increases intracellular calcium levels, which can activate calcium-dependent protein kinases. These kinases can phosphorylate SRRM5, resulting in its functional activation due to changes in its phosphorylation state. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA functions as a diacylglycerol analog, activating protein kinase C (PKC). PKC can phosphorylate serine and threonine residues on SRRM5, leading to its activation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is an inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A), which prevents dephosphorylation of proteins. Inhibition of these phosphatases can lead to the sustained phosphorylation and consequent activation of SRRM5. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to Okadaic Acid, Calyculin A is also an inhibitor of PP1 and PP2A, leading to increased phosphorylation levels of proteins in the cell. This can contribute to the phosphorylation and activation of SRRM5. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that selectively transports calcium ions across cell membranes, thus increasing intracellular calcium concentration. Elevated calcium can activate calcium-sensitive signaling pathways that lead to the phosphorylation and activation of SRRM5. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor, leading to an increase in cytosolic calcium levels. The rise in calcium can activate downstream kinases that can phosphorylate and activate SRRM5. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl cAMP is a cell-permeable cAMP analog that activates PKA. Activated PKA can phosphorylate SRRM5, resulting in its activation. | ||||||
Phorbol | 17673-25-5 | sc-253267 | 5 mg | $270.00 | 1 | |
4-Phorbol 12,13-didecanoate acts similarly to PMA, as a PKC activator. PKC activation can lead to the phosphorylation and activation of SRRM5. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that activates stress-activated protein kinases (SAPKs), also known as JNKs. JNK activation can lead to the phosphorylation of various substrates, possibly including SRRM5, resulting in its activation. | ||||||
Cyclosporin A | 59865-13-3 | sc-3503 sc-3503-CW sc-3503A sc-3503B sc-3503C sc-3503D | 100 mg 100 mg 500 mg 10 g 25 g 100 g | $63.00 $92.00 $250.00 $485.00 $1035.00 $2141.00 | 69 | |
Cyclosporin A binds to the cytosolic protein cyclophilin of immunocompetent lymphocytes, especially T-lymphocytes. This complex of cyclosporin A and cyclophilin inhibits calcineurin, which is a protein phosphatase. By inhibiting calcineurin, Cyclosporin A can indirectly lead to the increased phosphorylation and activation of downstream proteins such as SRRM5. | ||||||