RBM19 Inhibitors

Chemical inhibitors of RBM19 can exert their effects through various mechanisms that impede different aspects of cellular function. Paclitaxel, for instance, stabilizes microtubules and prevents their disassembly, affecting mitotic spindle formation and, consequently, cell cycle progression. This disruption can impact the function of RBM19, especially considering its role in nucleolar organization and ribosome biogenesis. Similarly, Camptothecin, by inhibiting Topoisomerase I, induces DNA damage during replication, which can influence RBM19's involvement in ribosomal RNA processing. Actinomycin D, known for its ability to bind to DNA at the transcription initiation complex, inhibits RNA elongation by RNA polymerase, thereby potentially affecting rRNA synthesis and, indirectly, RBM19's function in ribosome formation.

Aphidicolin, an inhibitor of DNA polymerases α and δ, halts DNA replication, which can disrupt cell cycle progression and indirectly inhibit the nucleolar activities of RBM19. Etoposide, which targets Topoisomerase II, leads to DNA strand breaks, potentially influencing RBM19's role due to the cellular response to DNA damage. Mitomycin C, by forming crosslinks within DNA, can also cause replication fork collapse, indirectly affecting RBM19's function in ribosome biogenesis. Brefeldin A disrupts transport between the endoplasmic reticulum and Golgi apparatus, leading to a loss of Golgi function, which can impede RBM19's role in ribosome assembly. Rocaglamide, by inhibiting eIF4A, can decrease protein synthesis demand, which may reflect on the necessity for RBM19's ribosome biogenesis activity. Cyclosporin A's inhibition of calcineurin affects NFAT, a transcription factor that regulates genes involved in ribosome biogenesis, with potential implications for RBM19. Rapamycin's inhibition of mTOR signaling, which is crucial for cell growth and ribosome biogenesis, can likewise affect RBM19's function. U0126, by inhibiting MEK1/2 and thus the MAPK/ERK pathway, can indirectly influence RBM19's role in ribosome production. Lastly, Leptomycin B targets the nuclear export of proteins by binding to exportin 1, which could result in the nuclear accumulation of ribosomal proteins and RNAs, disrupting RBM19's functions associated with ribosome biogenesis.