Date published: 2025-9-18

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pH Indicators

Santa Cruz Biotechnology now offers a broad range of pH Indicators for use in various applications. pH indicators are vital chemical compounds that change color in response to changes in pH, providing a visual representation of the acidity or alkalinity of a solution. In scientific research, these indicators are crucial for experiments requiring precise pH conditions, enabling researchers to maintain and verify the pH of environments in biochemical and chemical reactions. The utility of pH indicators extends across disciplines, including chemistry, environmental science, and molecular biology, where they serve as essential tools for monitoring reactions, studying enzyme activities, and investigating metabolic pathways. The ability to quickly assess pH values with indicators aids in the optimization of conditions for experimental protocols and ensures the reproducibility of results. These indicators are especially important in studies involving biochemical systems where pH can influence the activity and stability of molecules like proteins and nucleic acids. Advances in pH indicator technology have led to the development of more sensitive and selective indicators that can provide real-time, quantifiable data essential for detailed scientific analysis. The diversity of available pH indicators, each with unique transition pH ranges and colors, allows for their application in a variety of experimental scenarios, supporting a broad range of scientific inquiries. These compounds are integral to the toolkit of researchers aiming to explore and manipulate the chemical foundations of biological processes. View detailed information on our available pH Indicators by clicking on the product name.

Items 1 to 10 of 54 total

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Product NameCAS #Catalog #QUANTITYPriceCitationsRATING

Alizarin Red S

130-22-3sc-205998
sc-205998A
sc-205998B
sc-205998C
25 g
100 g
250 g
1 kg
$92.00
$200.00
$465.00
$1230.00
17
(1)

Alizarin Red S is a synthetic dye that exhibits remarkable pH-dependent behavior, transitioning between distinct colors in acidic and alkaline environments. This chromatic shift is attributed to its ability to form hydrogen bonds and engage in protonation-deprotonation reactions, altering its electronic structure. The compound's chelating properties allow it to form stable complexes with metal ions, influencing its solubility and reactivity in various chemical contexts. Its unique structural features contribute to its distinct optical characteristics, making it a valuable tool for studying pH variations.

Methyl orange

547-58-0sc-206030
sc-206030A
sc-206030B
25 g
100 g
250 g
$28.00
$51.00
$112.00
3
(0)

Methyl orange is a pH indicator known for its vivid color change from red in acidic solutions to yellow in neutral to alkaline conditions. This transition occurs due to the protonation state of its azo group, which affects its electronic configuration and light absorption properties. The compound's rapid response to pH changes is linked to its low molecular weight and the stability of its ionic form, making it an effective tool for monitoring acidity in various environments.

Congo Red

573-58-0sc-359843
sc-359843A
sc-359843B
sc-359843C
25 g
100 g
250 g
1 kg
$36.00
$87.00
$178.00
$495.00
4
(2)

Congo Red is a pH indicator that exhibits a striking color shift from blue in alkaline conditions to red in acidic environments. This behavior is attributed to its unique azo linkage, which undergoes tautomeric shifts that alter its electronic structure and light absorption. The compound's dual ionic and molecular forms contribute to its sensitivity, allowing for quick detection of pH changes. Its distinct interactions with hydrogen ions enhance its utility in various analytical applications.

Neutral Red Solution

553-24-2sc-281691
100 ml
$50.00
9
(1)

Neutral Red Solution is a pH indicator characterized by its vibrant color transition from red in acidic conditions to yellow in neutral to alkaline environments. This behavior arises from its unique structure, which allows for protonation and deprotonation processes that influence its electronic properties. The compound's ability to form hydrogen bonds enhances its sensitivity to pH fluctuations, making it an effective tool for monitoring subtle changes in acidity.

BCECF/AM

117464-70-7sc-202492
1 mg
$121.00
6
(1)

BCECF/AM is a fluorescent pH indicator that exhibits a distinct emission shift in response to changes in hydrogen ion concentration. Its unique molecular structure allows for selective protonation, leading to alterations in fluorescence intensity. This compound's hydrophobic characteristics enable it to permeate cell membranes, where it can undergo hydrolysis to release the active BCECF form, facilitating real-time pH monitoring in various environments.

Phenol Red

143-74-8sc-203752
sc-203752A
sc-203752B
5 g
25 g
250 g
$36.00
$85.00
$311.00
3
(1)

Phenol Red is a pH indicator that undergoes a notable color change in response to variations in acidity. Its unique structure features a phenolic hydroxyl group, which participates in proton transfer reactions, allowing it to act as a weak acid. This compound exhibits distinct spectral properties, with absorbance shifting from yellow in acidic conditions to red in alkaline environments. Its solubility in aqueous solutions enhances its utility in diverse chemical analyses, providing clear visual cues for pH assessment.

2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein

85138-49-4sc-202883
1 mg
$141.00
3
(0)

2',7'-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein is a highly sensitive pH indicator characterized by its unique fluorescence properties. This compound features multiple carboxylic acid groups that facilitate protonation and deprotonation, leading to significant shifts in its emission spectrum. Its ability to form hydrogen bonds enhances its interaction with surrounding molecules, resulting in distinct fluorescence changes across varying pH levels, making it an effective tool for monitoring acidic and basic environments.

8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt

27928-00-3sc-291738
1 g
$117.00
3
(1)

8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt, exhibits remarkable solubility and stability in aqueous solutions, making it an effective pH-sensitive probe. Its sulfonic acid groups contribute to strong ionic interactions, allowing for rapid proton exchange and significant shifts in optical properties. The compound's unique structure enables it to engage in π-π stacking and electrostatic interactions, enhancing its responsiveness to pH changes and facilitating dynamic environmental assessments.

Neutral Red

553-24-2sc-203748
sc-203748A
50 g
250 g
$108.00
$151.00
2
(2)

Neutral Red is a versatile pH indicator characterized by its unique ability to undergo reversible protonation, which alters its color in response to pH fluctuations. The compound features a distinct aromatic structure that allows for strong hydrogen bonding and hydrophobic interactions, enhancing its sensitivity to changes in acidity. Its dynamic equilibrium between protonated and deprotonated forms results in notable shifts in absorbance, making it an effective tool for monitoring pH variations in diverse environments.

Bromocresol Green sodium salt

62625-32-5sc-206036
sc-206036A
25 g
100 g
$153.00
$363.00
(1)

Bromocresol Green sodium salt is a pH indicator distinguished by its dual colorimetric response, transitioning from yellow in acidic conditions to blue in alkaline environments. This compound exhibits strong ionic interactions due to its sulfonate groups, which enhance solubility in aqueous solutions. Its unique structural features allow for rapid electron transfer, facilitating swift color changes that reflect minute pH variations, making it an effective choice for precise pH monitoring.