MAP LC3γ Inhibitors
The chemical class of MAP LC3γ Inhibitors would encompass compounds that modify the activity of the MAP LC3γ protein. Given that MAP LC3γ is involved in autophagy, inhibitors in this category would likely act by modulating the autophagic process. This modulation can occur at different stages of autophagy, including the initiation phase, autophagosome nucleation, autophagosome elongation, autophagosome maturation, and the final degradation phase within lysosomes. Compounds like 3-methyladenine, wortmannin, LY294002, and SAR405 target the early stages of autophagy, impacting the formation of autophagosomes where MAP LC3γ plays a role. These compounds inhibit the activity of class III PI3K (Vps34), which is critical for the initiation of autophagy. By inhibiting this kinase, the formation of autophagosomes is reduced, and consequently, the activity of MAP LC3γ in autophagosome formation is also reduced.
On the other hand, compounds such as chloroquine, hydroxychloroquine, and Lys05 disrupt the later stages of autophagy by preventing the acidificationof lysosomes. This acidification is crucial for the fusion between autophagosomes and lysosomes, a process necessary for the degradation of autophagic substrates. As a result, these inhibitors cause an accumulation of MAP LC3γ due to the blocking of autophagic flux. Similarly, Bafilomycin A1 also hinders the fusion of autophagosome and lysosome by specifically inhibiting V-ATPase, leading to an increase in the levels of MAP LC3γ associated with autophagosomes. Other inhibitors like Spautin-1, E64d, and NH4Cl affect proteins responsible for the degradation of autophagic components within lysosomes. Spautin-1 indirectly decreases MAP LC3γ levels by promoting the degradation of proteins related to autophagy through the inhibition of deubiquitinating enzymes USP10 and USP13. E64d and NH4Cl, on the other hand, inhibit lysosomal proteases and raise lysosomal pH, respectively, both leading to impaired degradation of autophagic vesicles and thereby causing an accumulation of MAP LC3γ.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $69.00 | 2 | |
Chloroquine can inhibit autophagy by preventing the acidification of lysosomes, which is necessary for autophagosome fusion and degradation, thereby potentially increasing MAP LC3γ levels due to reduced turnover. | ||||||
Autophagy Inhibitor, 3-MA | 5142-23-4 | sc-205596 sc-205596A | 50 mg 500 mg | $65.00 $261.00 | 113 | |
This compound can inhibit autophagy through its action as a PI3K inhibitor, which is necessary for autophagosome formation, potentially leading to decreased MAP LC3γ association with autophagic vesicles. | ||||||
Bafilomycin A1 | 88899-55-2 | sc-201550 sc-201550A sc-201550B sc-201550C | 100 µg 1 mg 5 mg 10 mg | $98.00 $255.00 $765.00 $1457.00 | 280 | |
Bafilomycin A1 can inhibit the vacuolar-type H+-ATPase (V-ATPase), preventing autophagosome-lysosome fusion, which can result in increased MAP LC3γ levels due to reduced degradation. | ||||||
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $67.00 $223.00 $425.00 | 97 | |
Wortmannin is a PI3K inhibitor that can impede the initiation of autophagy, possibly leading to reduced MAP LC3γ participation in autophagosome formation. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
As a PI3K inhibitor, LY294002 can interfere with autophagy initiation and autophagosome formation, potentially affecting MAP LC3γ activity. | ||||||
Spautin-1 | 1262888-28-7 | sc-507306 | 10 mg | $168.00 | ||
Spautin-1 can promote the degradation of autophagy-related proteins by inhibiting USP10 and USP13, potentially diminishing MAP LC3γ levels. | ||||||
hydroxychloroquine | 118-42-3 | sc-507426 | 5 g | $57.00 | 1 | |
Similar to chloroquine, hydroxychloroquine can disrupt lysosomal acidification and autophagosome degradation, which may lead to increased MAP LC3γ due to reduced degradation. | ||||||
Vinblastine | 865-21-4 | sc-491749 sc-491749A sc-491749B sc-491749C sc-491749D | 10 mg 50 mg 100 mg 500 mg 1 g | $102.00 $235.00 $459.00 $1749.00 $2958.00 | 4 | |
This alkaloid can disrupt microtubules and affect autophagosome trafficking, which can alter MAP LC3γ distribution and function. | ||||||
E-64 | 66701-25-5 | sc-201276 sc-201276A sc-201276B | 5 mg 25 mg 250 mg | $281.00 $947.00 $1574.00 | 14 | |
E64d is a lysosomal protease inhibitor that can prevent the breakdown of autophagosomal contents, potentially leading to increased MAP LC3γ levels. | ||||||
FCM Lysing solution (1x) | sc-3621 | 150 ml | $62.00 | 8 | ||
NH4Cl raises the lysosomal pH, which can inhibit the degradation phase of autophagy, potentially resulting in the accumulation of MAP LC3γ. | ||||||