CCDC28B Activators
The chemical class of CCDC28B Activators would refer to a group of compounds that enhance the activity of the protein encoded by the CCDC28B gene. Assuming such a class exists, the initial step in identifying these activators would involve deploying high-throughput screening (HTS) techniques. This process screens vast libraries of chemical compounds to discover molecules that can upregulate the activity of CCDC28B. These screenings typically utilize cell-based or in vitro assays that can detect changes in protein function, often through fluorescent or luminescent reporters that are responsive to the protein's activity levels. Compounds that produce a measurable and reproducible increase in the signal corresponding to the protein's activity are earmarked for further study. Following this, secondary assays are conducted to confirm the specificity of the compounds' effects on CCDC28B.
After identifying potential activators, the next phase involves understanding the interaction between these molecules and the CCDC28B protein. Structural biology techniques, such as X-ray crystallography or cryo-electron microscopy, may be used to visualize the binding of activators to the protein at an atomic level. This information is invaluable as it allows for the identification of key binding sites and the conformational changes in the protein that result in increased activity. Concurrently, biophysical assays, such as surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC), are employed to quantify the binding kinetics and affinity of the activators to the protein. These studies offer insights into how quickly and strongly the activators associate with the protein. Additionally, structure-activity relationship (SAR) studies are implemented to optimize the activator's chemical structure for better efficacy and selectivity. Chemists synthesize analogs of the active compounds, methodically altering parts of the molecule and measuring the resulting changes in CCDC28B activity.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin is a diterpene that functions as an adenylyl cyclase activator, thus increasing levels of cyclic AMP (cAMP) in cells. Elevated cAMP enhances protein kinase A (PKA) activity, which can phosphorylate various substrates that may interact with CCDC28B, influencing its role in ciliogenesis and cytoskeletal organization by altering the cellular localization and function of CCDC28B-associated proteins. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX (3-Isobutyl-1-methylxanthine) acts as a non-selective inhibitor of phosphodiesterases, leading to an increase in intracellular cAMP by preventing its breakdown. This rise in cAMP can activate PKA, which may modulate substrates within the ciliogenesis pathways, thereby indirectly affecting CCDC28B's function in the formation and maintenance of cilia. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $75.00 $212.00 | 18 | |
Rolipram is a selective phosphodiesterase-4 inhibitor that raises intracellular cAMP levels. By increasing cAMP, Rolipram indirectly activates PKA, which may phosphorylate proteins that interact with or regulate CCDC28B, potentially enhancing CCDC28B's role in ciliary functions and cytoskeletal dynamics. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A), which prevents dephosphorylation of proteins. By inhibiting PP1 and PP2A, phosphorylation states of proteins involved in ciliogenesis may be maintained, potentially supporting CCDC28B's function in the formation and stability of cilia by modulating the phosphorylation status of associated proteins or CCDC28B itself. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Calyculin A, like Okadaic acid, is an inhibitor of protein phosphatases PP1 and PP2A. By inhibiting these phosphatases, Calyculin A can maintain the phosphorylation of ciliary proteins, which may indirectly enhance the function of CCDC28B in ciliary assembly and maintenance by altering the phosphorylation dynamics within ciliary pathways. | ||||||
Adenosine 3′,5′-cyclic monophosphate | 60-92-4 | sc-217584 sc-217584A sc-217584B sc-217584C sc-217584D sc-217584E | 100 mg 250 mg 5 g 10 g 25 g 50 g | $114.00 $175.00 $260.00 $362.00 $617.00 $1127.00 | ||
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates PKA, leading to phosphorylation of substrates involved in ciliogenesis. Enhanced PKA activity due to db-cAMP can indirectly augment the function of CCDC28B in ciliary structure and function. | ||||||