C11orf35 Activators
C11orf35 engage distinct cellular pathways to modulate its function. Forskolin, by elevating intracellular cAMP levels, leads to the activation of protein kinase A (PKA). The activation of PKA can result in the phosphorylation of C11orf35, altering its activity or localization within the cell. Similarly, dibutyryl-cAMP and 8-Br-cAMP, both analogs of cAMP, also activate PKA, potentially phosphorylating C11orf35. On a related note, H-89, despite being a PKA inhibitor, may indirectly cause PKA activation through feedback loops, resulting in C11orf35 phosphorylation. In another pathway, PMA activates protein kinase C (PKC), which is known to phosphorylate a broad spectrum of proteins. PKC activation can lead to phosphorylation of C11orf35, modifying its conformation and function.
Intracellular calcium plays a critical role in the activation of C11orf35 through the actions of ionomycin and thapsigargin. Ionomycin, a calcium ionophore, increases the intracellular concentration of calcium, which can activate calmodulin-dependent kinases that phosphorylate C11orf35. Thapsigargin, by inhibiting the SERCA pump, also raises cytosolic calcium levels, potentially leading to C11orf35 activation via similar calcium-dependent phosphorylation events. On the other hand, epidermal growth factor (EGF) engages its receptor, triggering downstream signaling cascades such as the MAPK/ERK pathway, which may phosphorylate and activate C11orf35. Insulin initiates a cascade through its receptor, leading to PI3K/AKT pathway activation and subsequent phosphorylation of various substrates, including possibly C11orf35. Additionally, anisomycin activates stress-activated protein kinases like JNK, which could phosphorylate and activate C11orf35. Lastly, both calyculin A and okadaic acid, through their inhibition of protein phosphatases, prevent the dephosphorylation of proteins, thereby maintaining C11orf35 in a phosphorylated, active state.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) activates protein kinase C (PKC), which can phosphorylate a multitude of proteins, potentially including C11orf35. PKC-mediated phosphorylation can change the conformation, activity, or localization of C11orf35, resulting in its functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinases, which could phosphorylate and thereby activate C11orf35. Activation of C11orf35 by this mechanism would involve calcium-dependent phosphorylation, altering its activity or interaction with other proteins. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin binds to its receptor, triggering receptor autophosphorylation and the activation of the PI3K/AKT signaling pathway. AKT can phosphorylate a wide range of substrates, potentially including C11orf35, leading to its functional activation through changes in its conformation, activity, or interaction with other cellular components. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is known to activate stress-activated protein kinases such as JNK. The activation of JNK could lead to the phosphorylation of C11orf35, resulting in a functional change that activates the protein. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels of many proteins. By inhibiting the dephosphorylation of C11orf35, it could maintain C11orf35 in an activated state. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Similar to calyculin A, okadaic acid is also an inhibitor of protein phosphatases. This inhibition can result in the hyperphosphorylation and subsequent activation of proteins, including possibly C11orf35. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates cAMP-dependent pathways, similar to forskolin. By increasing cAMP in the cell, it can lead to the activation of PKA, which may phosphorylate and activate C11orf35. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $108.00 $169.00 $295.00 $561.00 $835.00 | 2 | |
8-Bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) is a synthetic analogue of cAMP that activates PKA. Activation of PKA can lead to phosphorylation of various proteins, potentially including C11orf35, leading to its activation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor that leads to an increase in cytosolic calcium levels. Elevated calcium may activate calmodulin-dependent kinases or other calcium-sensitive proteins that can phosphorylate and activate C11orf35. | ||||||