Vitamin D Receptor/VDR抗体 (D-6) 推荐用于 mouse, rat 和 human 来源的VDR WB, IP, IF 和 IHC(P)检测

Vitamin D Receptor/VDR 抗体 (D-6): sc-13133

Vitamin D Receptor/VDR抗体 (D-6) is rated 4.6 out of 5 by 30.
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说明书

    • 抗-Vitamin D Receptor/VDR 抗体 D-6 是小鼠单克隆 IgG2a κ,Vitamin D Receptor/VDR抗体, 在344篇文献中引用,规格为200 µg/ml
    • 免疫human物种的VDR的氨基酸344-424
    • 抗-Vitamin D Receptor/VDR 抗体 (D-6) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 VDR
    • 抗-Vitamin D Receptor/VDR 抗体 (D-6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
    • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13133 X, 200 µg/0.1 ml)
    • 关于如何获取Vitamin D Receptor/VDR (D-6): sc-13133免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) 是 Vitamin D Receptor/VDR Antibody (D-6) for WB and IHC(P) 的首选应用二抗。这个产品以套装形式提供 Vitamin D Receptor/VDR Antibody (D-6) (参照下方订购信息).

Hi there, since the VDR has different isoforms, I am wondering the amino acids sequence for VDR this antibody corresponding. Thanks!

Asked by: Fay Liu
Thank you for your question. VDR Antibody (D-6): sc-13133 was raised against amino acids 344-424 of VDR isoform 1 of human origin. This is the corresponding amino acid sequence: PGVQDAA LIEAIQDRLS NTLQTYIRCR HPPPGSHLLY AKMIQKLADL RSLNEEHSKQ YRCLSFQPEC SMKLTPLVLE VFGN Please do not hesitate to contact our technical service directly if you have additional questions.
Answered by: Tech Support Europe
Date published: 2019-06-05

does D6 vitamin d receptor antibody cross react with monkey (macaques) for immunohistochemistry

Asked by: schristak
Thank you for your question. This antibody has not been tested on monkey samples in house, so it is not technically recommended or warrantied for that use, however, we do have publications in monkey (rhesus) samples.
Answered by: Tech Service
Date published: 2019-04-09

Hello, I have been working with another VDR and I am not certain I am fully appreciating that VDR. Hence I am interested in the "Contact us to receive a FREE 10 µg sample of VDR (D-6): sc-13133." offer of getting 10 ug for free. Is it possible?

Asked by: hanna abrahamsson
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Can Santa Cruz provide VDR IHC images (performed using D-6 sc-13133 ab) with staining intensity (score) of 1 to 4 and % positive stained cells (score) with 1-4 (preferably in FFPE colorectal tumor tissue samples) ?

Asked by: Sanbc
Thank you for the question. Please note that this product is sold for research or laboratory use only and it must not been used for medical diagnostics. All available IHC(P) images for this product will be published upon availability on our product website. We currently show staining of human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells, human skin tissue showing nuclear and cytoplasmic staining of keratinocytes, fibroblasts, Langerhans cells and melanocytes and human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Answered by: Technical Support Europe
Date published: 2018-01-10

I got a double band when I used this antibody, any idea what the other band could be? Thanks

Asked by: Reham
Thank you very much for your inquiry. Please understand that we do not interpret customer data, since we do not have a detailed knowledge about your experiment and how the data was produced. Therefore our interpretation of the data could lead to a wrong direction without knowing all the relevant details. However, Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Common factors include: 1. Post-translational modification - e.g. phosphorylation, glycosylation etc, which increases the size of the protein. 2. Post-translation cleavage - e.g. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. pro-caspases. 3. Splice variants - alternative splicing may create different sized proteins produced from the same gene. 4. Relative charge - the composition of amino acids (charged vs non-charged). 5. Multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
Answered by: Technical Support Europe
Date published: 2018-09-01

What is VDR D-6 best diluted in?

Asked by: Daraaaa
Thank you for your question. What it is best diluted in depends on the application you plan on using it for. For Western blot, we would recommend diluting it in 5% milk with TBS-T. For IF/IHC, we would recommend diluting it in blocking serum. You can find more information in the protocol you plan on following: https://www.scbt.com/scbt/resources/protocols
Answered by: Tech Service
Date published: 2017-05-25

Hello, Does this ab work fine when it is conjugated to a regular anti-mouse IgG? Thanks!

Asked by: MMac
Thank you for your question. Yes, this antibody can be used with a conventional anti-mouse IgG secondary or our Mouse IgG Binding Protein, both are appropriate.
Answered by: Technical Service
Date published: 2017-04-24

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-13133 is available directly conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2021-01-21
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Rated 5 out of 5 by from Worked well in human and mouse muscle tissue. I used this antibody for IHC and got good results following published protocols. I used the unconjugated primary antibody and had to amplify for the human samples, but the mouse samples worked well going straight into secodary.
Date published: 2021-01-25
Rated 3 out of 5 by from low dilution I needed to use a low dilution to make it work (1:50)
Date published: 2020-09-22
Rated 5 out of 5 by from Buen anticuerpo Tiene una alta especificidad, en wblot da una muy buena señal y se puede reutilizar varias veces.
Date published: 2020-09-01
Rated 5 out of 5 by from very good antibody This antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Date published: 2019-01-02
Rated 5 out of 5 by from Best Antibody We have tried this antibody and published. Instruction and protocol was well described. I strongly recommend this antibody to other researcher.
Date published: 2018-08-24
Rated 5 out of 5 by from EXCELLENT SENSITIVITY TO SEE VDR ON PROTEIN LEVEL IS VERY TOUGH, BUT USING THE HYPEROSMOLAR BUFFER LYSIS AND THIS VDR ANTIBODY. I GOT THE BAND OF VDR. THIS ANTIBODY IS BETTER THAN ABCAM. THANK YOU
Date published: 2018-04-12
Rated 5 out of 5 by from Works well for immunofluorescence and wester blot This antibody works very well at 1:1000 dilution for western blot and at 1:200 dilution for immunofluorescence on mouse calvariae.
Date published: 2018-03-16
Rated 4 out of 5 by from Arterial immunohistochemistry VDR antibody is a nuclear antibody, but the cytoplasm is also stained. Look at the pictures provided by manual also have cytoplasmic staining. Do you know how to explain properly?
Date published: 2017-10-16
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