TRAP150抗体 (F-10) 推荐用于 mouse, rat 和 human 来源的TRAP150 WB, IP, IF, IHC(P) 和 ELISA检测

TRAP150 抗体 (F-10): sc-133250

TRAP150抗体 (F-10) is rated 3.7 out of 5 by 3.
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说明书

    • 抗-TRAP150 抗体 F-10 是小鼠单克隆 IgG1 κ,TRAP150抗体, 在2篇文献中引用,规格为200 µg/ml
    • raised against amino acids 191-490 mapping within an internal region of TRAP150 of human origin
    • 抗-TRAP150 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TRAP150
    • 抗-TRAP150 抗体 (F-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于ChIP的TransCruz试剂(sc-133250 X, 200 µg/0.1 ml)
    • 关于如何获取TRAP150 (F-10): sc-133250免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 TRAP150 Antibody (F-10) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供TRAP150 Antibody (F-10)。 (见下方订购信息).
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Hello I am interested in the antibody against Thrap3 sc-133250 X. Since I am planning on using it in immunofluorescence on frozen sections as well as on ChIP, I wondered if you would recommend to purchase sc-133250 X for both applications? Many thanks

Asked by: Roberta M
Thank you for your question. The only difference between the conventional antibody product (sc-133250), and the TransCruz reagent (sc-133250 X), is that the TransCruz reagent is provided 10X more concentrated as 200 ug in 0.1 ml, in a buffer without any gelatin (as a stabilizing protein). If you would like to use the antibody for both immunofluorescence and ChIP then we would recommend purchasing the TransCruz reagent (sc-133250 X) because it could be used in either application. Please adjust your dilution(s) according to the more concentrated antibody. In addition, please note that this antibody has not yet been ChIP validated. The TransCruz primary antibody is recommended and warrantied for ChIP, however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-03

When should the TransCruz reagent sc-133250 X be used instead of the standard form sc-133250?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2019-01-03
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Rated 1 out of 5 by from Didn't work Antibody produced no bands on a western blot using neonatal rat ventricular myocytes.
Date published: 2017-10-04
Rated 5 out of 5 by from Robust signal in western blot analysis of Robust signal in western blot analysis of TRAP150 expression in K-562 nuclear extract. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Superb immunofluorescence nuclear staining Superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-06
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