Date published: 2025-9-9

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transferrin 抗体 (D-9): sc-365871

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说明书
  • transferrin抗体(D-9)是小鼠单克隆IgG1 λ transferrin抗体, 在9篇文献中引用,规格为200 µg/ml
  • 免疫human物种的transferrin的氨基酸326-390
  • transferrin 抗体 (D-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 transferrin
  • 抗transferrin抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取transferrin (D-9): sc-365871免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)是 transferrin 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与transferrin 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGλ BP 偶联产品,请参阅我们的 小鼠 IgG 结合蛋白完整列表。

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関連項目

transferrin 抗体(D-9)是一种 IgG1 λ 小鼠单克隆 transferrin 抗体(也称为粒细胞/花粉结合蛋白(GPBP)抗体、TFQTL1 抗体、76.5 kDa Transferrin 糖蛋白抗体、铁(Fe)转运糖蛋白抗体或载铁蛋白抗体),可通过 WB、IP、IF、IHC(P) 和 ELISA 检测人源 transferrin 蛋白。transferrin 抗体(D-9)既可以是非偶联的抗 transferrin 抗体形式,也可以是多种偶联形式的抗 transferrin 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。铁(Fe)是一种在所有活细胞中都存在的严格代谢控制的矿物质和生长因子。未与红细胞血红蛋白结合的铁由 transferrin(Tf)转运,transferrin 是脊椎动物血清中的铁转运蛋白。transferrin 蛋白包含两个同源结构域,每个结构域都含有一个 Fe 结合位点。大多数 transferrin 在肝脏中合成并分泌到血液中,但也在睾丸和大脑以及少突胶质细胞中少量产生,其中 transferrin 是少突胶质细胞分化的早期标志。在血液中,transferrin 通过与 transferrin 受体(TfR,也称为 CD71)结合,通过被覆凹坑和囊泡系统被红细胞和网织红细胞内化。释放 Fe 后,transferrin 返回细胞外介质,在那里它可以被重复使用。transferrin 基因的缺陷会导致无 transferrin 血症,这是一种罕见的常染色体隐性遗传病,其特征是小细胞性贫血和铁负荷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

transferrin 抗体 (D-9) 参考文献:

  1. 在人类少突胶质细胞系的分化过程中,替代剪接会阻止转铁蛋白的分泌。  |  de Arriba Zerpa, GA., et al. 2000. J Neurosci Res. 61: 388-95. PMID: 10931525
  2. 一个转铁蛋白血症病例的分子特征。  |  Beutler, E., et al. 2000. Blood. 96: 4071-4. PMID: 11110675
  3. 老年性黄斑变性的血清铁, 转铁蛋白饱和度, 铁蛋白和饮食数据。  |  Richer, S., et al. 2002. Am J Ther. 9: 25-8. PMID: 11782816
  4. 转铁蛋白基因表达调控。  |  Zakin, MM. 1992. FASEB J. 6: 3253-8. PMID: 1426763
  5. [转铁蛋白受体在红细胞生成过程中的表达和细胞外释放]  |  Kohgo, Y., et al. 1991. Rinsho Ketsueki. 32: 580-6. PMID: 1890732
  6. 缺氧时的转铁蛋白糖基化  |  Regoeczi, E., et al. 1991. Biochem Cell Biol. 69: 239-44. PMID: 2054155
  7. 转铁蛋白依赖性脂质过氧化  |  Saito, M., et al. 1986. J Free Radic Biol Med. 2: 99-105. PMID: 3029212
  8. 肝细胞对铁的吸收和新陈代谢  |  Morgan, EH. and Baker, E. 1986. Fed Proc. 45: 2810-6. PMID: 3533647
  9. 转铁蛋白介导的细胞铁吸收。  |  Irie, S. and Tavassoli, M. 1987. Am J Med Sci. 293: 103-11. PMID: 3551609
  10. 人类转铁蛋白:cDNA 特征和染色体定位。  |  Yang, F., et al. 1984. Proc Natl Acad Sci U S A. 81: 2752-6. PMID: 6585826
  11. 人血清转铁蛋白的一级结构。七个溴化氰片段的结构和完整结构的组装。  |  MacGillivray, RT., et al. 1983. J Biol Chem. 258: 3543-53. PMID: 6833213
  12. 转铁蛋白的结构和功能  |  Aisen, P. and Brown, EB. 1975. Prog Hematol. 9: 25-56. PMID: 766075

订购信息

产品名称产品编号规格价格数量收藏夹

transferrin 抗体 (D-9)

sc-365871
200 µg/ml
$316.00

transferrin (D-9): m-IgGλ BP-HRP 套装

sc-522402
200 µg Ab, 40 µg BP
$354.00

transferrin 抗体 (D-9) AC

sc-365871 AC
500 µg/ml, 25% agarose
$416.00

transferrin 抗体 (D-9) HRP

sc-365871 HRP
200 µg/ml
$316.00

transferrin 抗体 (D-9) FITC

sc-365871 FITC
200 µg/ml
$330.00

transferrin 抗体 (D-9) PE

sc-365871 PE
200 µg/ml
$343.00

transferrin 抗体 (D-9) Alexa Fluor® 488

sc-365871 AF488
200 µg/ml
$357.00

transferrin 抗体 (D-9) Alexa Fluor® 546

sc-365871 AF546
200 µg/ml
$357.00

transferrin 抗体 (D-9) Alexa Fluor® 594

sc-365871 AF594
200 µg/ml
$357.00

transferrin 抗体 (D-9) Alexa Fluor® 647

sc-365871 AF647
200 µg/ml
$357.00

transferrin 抗体 (D-9) Alexa Fluor® 680

sc-365871 AF680
200 µg/ml
$357.00

transferrin 抗体 (D-9) Alexa Fluor® 790

sc-365871 AF790
200 µg/ml
$357.00

Hello I would like to know how to store the antibody transferrin D9 after being diluted. Should I keep it in 4 degrees like the stock solution or should I keep it in - 20 degrees ? Thank you in advance 

Asked by: S Tlep
Thank you for your question. We do not recommend making aliquots of antibodies. The antibody and any dilutions you make should be kept at +4 °C. The buffer in which our antibodies are supplied keep them stable and they can easily last for several years under proper storage conditions. However, the buffer is not suitable for freezing the antibodies.
Answered by: Technical Support Europe
Date published: 2019-05-06

Hello How long should I incubate the western blot nitro cellulose membrane with transferrin antibody D-9, should it be overnight or 2 hours of incubation should be enough ? Thank you in advance 

Asked by: S Tlep
Thank you for your question. We start with a 1 hour at room temperature incubation, but if needed you can do an overnight incubation. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2019-04-29

This antibody (sc-365871) is an antibody against human transferrin. Does this antibody recognize mouse transferrin too? I have been looking for an antibody which binds mouse transferrin for IHP(P).

Asked by: kenji MI
Thank you for your question. We have not yet tested this antibody in mouse samples and so that is not a recommended species at this time. We would recommend using one of our mouse-reactive transferrin antibodies (sc-373785 or sc-52256) which are recommended for IF or IHC on fresh or frozen tissues or cells fixed in acetone or methanol. Those antibodies have not yet been tested in formalin-fixed paraffin embedded tissue sections, but they may also be suitable for your application.
Answered by: Technical Support
Date published: 2018-12-21

Can transferrin (D-9): sc-365871 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, transferrin (D-9): sc-365871 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good AntibodyWhen testing, we saw some nonspecific banding but expected size was clear and detectable.
Date published: 2021-11-22
Rated 3 out of 5 by from weak band in SiHa cellsThis antibody recognizes a very weak band in SiHa cell line (78 kDa, dil 1:200) by western blot experiments performed using total cellular extract (50 ug).
Date published: 2021-01-26
Rated 4 out of 5 by from It is great.It's still a good loading control for serum proteins, though the backgrounds are not very clean.
Date published: 2017-11-28
Rated 5 out of 5 by from Monkey serumI purchased this antibody for westernblotting of rhesus serum.
Date published: 2017-08-17
Rated 5 out of 5 by from 可以这个抗体很好,我用的rat来源的细胞样品,条带出现在70-100之间,稀释倍数500,背景干净,不错
Date published: 2017-07-17
Rated 5 out of 5 by from Conjugated to HRP and got very clean WesternConjugated to HRP and got very clean Western Blot images.
Date published: 2015-03-10
Rated 5 out of 5 by from Good Western Blot data of transferrin expressionGood Western Blot data of transferrin expression in Hep G2 whole cell lysate and human kidney and human liver tissue extracts. -SCBT QC
Date published: 2015-01-20
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transferrin 抗体 (D-9) is rated 4.6 out of 5 by 7.
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