TGFβ Receptor II/TGFBR2抗体 (C-4) 推荐用于 mouse, rat 和 human 来源的TGFβ RII WB, IP, IF, IHC(P) 和 ELISA检测

TGFβ Receptor II/TGFBR2 抗体 (C-4): sc-17791

TGFβ Receptor II/TGFBR2抗体 (C-4) is rated 3.4 out of 5 by 26.
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说明书

    • 抗-TGFβ Receptor II/TGFBR2 抗体 C-4 是小鼠单克隆 IgG1 κ,TGFβ Receptor II/TGFBR2抗体, 在28篇文献中引用,规格为200 µg/ml
    • raised against amino acids 1-567 representing full length TFGβ RII of human origin
    • 抗-TGFβ Receptor II/TGFBR2 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TGFβ RII
    • 抗-TGFβ Receptor II/TGFBR2 抗体 (C-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取TGFβ Receptor II/TGFBR2 (C-4): sc-17791免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 TGFβ Receptor II/TGFBR2 Antibody (C-4) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供TGFβ Receptor II/TGFBR2 Antibody (C-4) (见下方订购信息).
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Is it fine to use TGFb RII antibody (C-4) for WB in lysates from human PBMCs (RIPA buffer), in loading buffer Laemmli+5% beta-mercapto, boiled at 95ºC for 5min?

Asked by: MCE91
Thank you for your message. Please find our highly recommended protocol for WB on the following link: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you have further questions, please do not hesitate to contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Can I use TGFb RII Antibody (C-4): sc-17791 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: sweetums
Yes. We recommend using a directly conjugated monoclonal antibody. NCAM (123C3): sc-7326 monoclonal antibody is available directly conjugated to either HRP (sc-17791 HRP), phycoerythrin (sc-17791 PE), fluorescein (sc-17791 FITC), Alexa Fluor® 488 (sc-17791 AF488), Alexa Fluor® 647 (sc-17791 AF647).
Answered by: Technical Support 13
Date published: 2018-07-10

Is it possible to use the AC conjugated TGFb RII Antibody (C-4): sc-17791 in both Western Blot and IP?

Asked by: sweetums
Thank you for your inquiry. We do not recommend the use of the AC conjugated form in Western blot. The AC conjugated form of the antibody is specifically used for IP. The unconjugated form of the antibody is the best choice when working in multiple applications.
Answered by: Technical Support 13
Date published: 2017-01-12
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Rated 4 out of 5 by from Good TGFBR2 antibody detected reasonably in human stromal cells by IF and IHC(P).
Date published: 2021-09-16
Rated 1 out of 5 by from Immunofluorescence I used the anti-TGF-beta receptor II antibody at 1:100 dilution. The sample (cultured epithelial cell) was fixed with 4% PFA followed by chilled methanol. Unfortunately, I could not detect this signal because of very low signal.
Date published: 2020-09-02
Rated 1 out of 5 by from Not recognizing the correct band I tested the free antibody sample from Santa Cruz with my TGFb RII wild type and knockout cell lysate. Unfortunately, this antibody did not recognize TGFb RII. Even though there were multiple bands on the blot, none of them show difference between WT and KO samples.
Date published: 2018-05-10
Rated 1 out of 5 by from TGFβ RII Antibody (C-4) I used whole heart homogenates for this WB. No bands showed up on the predicted size. Just a non-specific one at around 50kDa.
Date published: 2017-12-05
Rated 1 out of 5 by from Did not work for me Interestingly, TGFbeta RII (E-6) worked pretty well for my western blots. Not sure why this did not work.
Date published: 2017-11-06
Rated 5 out of 5 by from Recommended A clear band of WB was shown in the cell line. I chose 1/500 dilution, which was very satisfactory for this result.
Date published: 2017-10-25
Rated 4 out of 5 by from Good Though there are some none specific background, the main band is quite clear.
Date published: 2017-10-06
Rated 5 out of 5 by from Strong band It have a strong band in WB in hepg2 whole cell lysates.
Date published: 2017-09-20
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