Sodium phosphate (TSP) is water-soluble and widely used as a cleaning agent. The pH of a 1% solution is typically around 12, making this reagent sufficiently alkaline for saponification of grease and oils. Often used in the formulation of various buffers; complementary buffer components may include: Sodium phosphate monobasic (sc-215883; sc-251042), Sodium phosphate dibasic (sc-203277; sc-236917) or Potassium Phosphate, Dibasic (sc-203210). Trisodium phosphate is used in the isolation of mycobacteria, including culturing of tubercle bacilli. It has been used as a protein precipitant in certain applications and in co-precipitation and fractionization of nucleotides. Sodium phosphate may be used for the preservation of biologically active soil samples. Because of the alkaline pH, TSP disrupts cytoplasmic and outer membranes of Salmonella enterica cells making it useful in preventing the spread of bacteria.
Also available: Sodium phosphate tribasic dodecahydrate (sc-203403).
1. Armstrong, A.R. 1948. Can Med Assoc J. 59: 73. PMID: 18864250
2. Martin, M.E., et al. 1968. Appl Microbiol. 16: 506-508. PMID: 4967759
3. Chan, P.S. and Lin, M.C. 1974. Meth. Enzymol. 38: 38-41. PMID: 4375771
4. MacGregor, C.H. 1978. Meth. Enzymol. 53: 347-355. PMID: 362126
5. Sampathkumar, B., et al. 2003. Appl. Environ. Microbiol. 69: 122-129. PMID: 12513986
6. Rosell, M., et al. 2006. J Chromatogr A. 1132: 28-38. PMID: 16904119