Date published: 2025-9-7

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Rieske FeS 抗体 (A-5): sc-271609

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说明书
  • Rieske FeS 抗体 A-5 是小鼠单克隆 IgG1 κ,Rieske FeS抗体, 在13篇文献中引用,规格为200 µg/ml
  • 针对human起源的Rieske FeS内部区域内的氨基酸171-223进行
  • Rieske FeS 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rieske FeS
  • 抗Rieske FeS抗体(A-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rieske FeS (A-5): sc-271609免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Rieske FeS Antibody (A-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Rieske FeS Antibody (A-5) 打包提供(请参阅下面的订购信息)。

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関連項目

Rieske FeS抗体(A-5)是一种IgG1 κ小鼠单克隆Rieske FeS抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Rieske FeS蛋白。Rieske FeS抗体(A-5)既有非偶联抗Rieske FeS抗体形式,也有多种偶联形式的抗Rieske FeS抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞色素c是一种特征明确、可移动的电子传递蛋白,对所有需氧生物的能量转换至关重要。细胞色素b与细胞色素c亚基1和Rieske蛋白结合形成复合体III(也称为细胞色素bc1复合体),参与细胞呼吸。泛醌细胞色素c还原酶(UQCRFS1),也称为Rieske铁硫蛋白(Rieske FeS),是线粒体呼吸链复合体III的重要亚基。该复合体将电子从泛醌传递到细胞色素c。编码Rieske FeS的基因可能参与更具侵袭性乳腺癌表型的发展。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rieske FeS 抗体 (A-5) 参考文献:

  1. 急性髓性白血病和骨髓增生异常综合征中的双分染色体:通过荧光原位杂交和光谱核型鉴定新的扩增区。  |  Sait, SN., et al. 2002. Genes Chromosomes Cancer. 34: 42-7. PMID: 11921281
  2. 卵巢癌的 CA125 和 UQCRFS1 FISH 研究。  |  Kaneko, SJ., et al. 2003. Gynecol Oncol. 90: 29-36. PMID: 12821338
  3. 在线粒体内编码和合成的Rieske FeS蛋白可弥补核基因的缺陷。  |  Golik, P., et al. 2003. Proc Natl Acad Sci U S A. 100: 8844-9. PMID: 12837937
  4. 缺少Rieske FeS蛋白的罗得氏菌囊状体突变体形成稳定的细胞色素bc1亚复合物,并具有完整的醌还原位点。  |  Davidson, E., et al. 1992. Biochemistry. 31: 3351-8. PMID: 1313293
  5. 原发性乳腺癌核心活检样本中的泛醌细胞色素 c 还原酶 (UQCRFS1) 基因扩增。  |  Ohashi, Y., et al. 2004. Gynecol Oncol. 93: 54-8. PMID: 15047214
  6. 细胞色素b6f复合物腔域与叶绿素和细胞色素c6相互作用布朗动力学研究:Rieske FeS蛋白对相互作用的影响。  |  Haddadian, EJ. and Gross, EL. 2006. Biophys J. 91: 2589-600. PMID: 16844750
  7. 六味地黄汤对衰老加速小鼠海马基因表达的影响  |  Cheng, XR., et al. 2007. Fitoterapia. 78: 175-81. PMID: 17337329
  8. RieskeFeS蛋白的过度表达可提高电子传输速率和生物质产量。  |  Simkin, AJ., et al. 2017. Plant Physiol. 175: 134-145. PMID: 28754840
  9. 膜内Rieske FeS簇的分支杆菌琥珀酸脱氢酶结构。  |  Zhou, X., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33876763
  10. 人类 Rieske Fe-S 蛋白 UQCRFS1 基因的结构, 序列和位置。  |  Pennacchio, LA., et al. 1995. Gene. 155: 207-11. PMID: 7721092
  11. 将线粒体细胞色素 bc1 复合物 Rieske 铁硫蛋白亚基的基因(UQCRFS1)分配到人类基因组的 22q13 和 19q12-q13.1 区域。  |  Duncan, AM., et al. 1994. Genomics. 21: 281-3. PMID: 8088805
  12. 光刺激下,未组装的Rieske FeS蛋白被类囊体结合蛋白酶降解:FtsH蛋白酶的可能作用。  |  Ostersetzer, O. and Adam, Z. 1997. Plant Cell. 9: 957-65. PMID: 9212469

订购信息

产品名称产品编号规格价格数量收藏夹

Rieske FeS 抗体 (A-5)

sc-271609
200 µg/ml
$316.00

Rieske FeS (A-5): m-IgG Fc BP-HRP 套装

sc-529220
200 µg Ab; 10 µg BP
$354.00

Rieske FeS (A-5): m-IgGκ BP-HRP 套装

sc-521995
200 µg Ab, 40 µg BP
$354.00

Rieske FeS (A-5): m-IgG1 BP-HRP 套装

sc-543396
200 µg Ab; 20 µg BP
$354.00

Rieske FeS 抗体 (A-5) AC

sc-271609 AC
500 µg/ml, 25% agarose
$416.00

Rieske FeS 抗体 (A-5) HRP

sc-271609 HRP
200 µg/ml
$316.00

Rieske FeS 抗体 (A-5) FITC

sc-271609 FITC
200 µg/ml
$330.00

Rieske FeS 抗体 (A-5) PE

sc-271609 PE
200 µg/ml
$343.00

Rieske FeS 抗体 (A-5) Alexa Fluor® 488

sc-271609 AF488
200 µg/ml
$357.00

Rieske FeS 抗体 (A-5) Alexa Fluor® 546

sc-271609 AF546
200 µg/ml
$357.00

Rieske FeS 抗体 (A-5) Alexa Fluor® 594

sc-271609 AF594
200 µg/ml
$357.00

Rieske FeS 抗体 (A-5) Alexa Fluor® 647

sc-271609 AF647
200 µg/ml
$357.00

Rieske FeS 抗体 (A-5) Alexa Fluor® 680

sc-271609 AF680
200 µg/ml
$357.00

Rieske FeS 抗体 (A-5) Alexa Fluor® 790

sc-271609 AF790
200 µg/ml
$357.00

Could you provide some research publication in scientific journals showing the use of A-5 in tissue staining experiments? Thank you!

Asked by: Lan zhou
Thank you for your question. We are not currently aware of any product citations using this antibody for IHC or immunohistochemistry applications. We have in-house data published on our website showing staining in FFPE human kidney, rectum, colon and skeletal muscle tissue.
Answered by: Technical Support
Date published: 2019-04-13

I am using Rieske FeS (A-5): sc-271609 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well for both IF and westernStrong and specific labeling for both western and immunoifluorescence. Great mitochondrial marker antibody (tried in 4% PFA fixed HeLa cells).
Date published: 2017-06-27
Rated 4 out of 5 by from Good antibody, could be a bit cleaner for westernAntibody gives a nice strong band for western blotting, but there are some other lighter bands that appear at a lower molecular weight as well.
Date published: 2017-06-27
Rated 4 out of 5 by from We used it in IF and obtained publishable resultsWe used it in IF and obtained publishable results.
Date published: 2015-09-01
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-17
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Rieske FeS 抗体 (A-5) is rated 4.5 out of 5 by 4.
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