Date published: 2025-9-5
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PP2A-Cα/β Antibody (1D6) - Western Blotting - Image 368015
PP2A-Cα/β 抗体 (1D6) HRP: sc-80665 HRP. NIH/3T3 (A), A-673 (B), MCF7 (C), HeLa (D), U-937 (E) 和 Hep G2 (F) 全细胞裂解液中 PP2A-Cα/β 表达的直接 Western 印迹分析.
PP2A-Cα/β 抗体 (1D6) HRP: sc-80665 HRP. NIH/3T3 (A), A-673 (B), MCF7 (C), HeLa (D), U-937 (E) 和 Hep G2 (F) 全细胞裂解液中 PP2A-Cα/β 表达的直接 Western 印迹分析.

PP2A-Cα/β 抗体 (1D6): sc-80665

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寫評論提問

说明书
  • PP2A-Cα/β抗体(1D6)是小鼠单克隆IgG2b κ, 在34篇文献中引用，规格为100 µg/ml
  • 针对human PP2A的PP2A-Cα/β的氨基酸295-309
  • PP2A-C alpha / beta 抗体 (1D6) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human, bovine, porcine, canine 和 bacterial来源的PP2A-Cα/β; 脱甲基化，在冰上用100mM NaOH处理
  • 抗PP2A-C alpha / beta抗体（1D6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PP2A-Cα/β (1D6): sc-80665的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 PP2A-Cα/β 抗体 (1D6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PP2A-Cα/β 抗体 (1D6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PP2 抗体包括 PP2A-A α, PP2A-A alpha / β, PP2A-B55, PP2A-B55-α, PP2A-B55-γ, PP2A-B56-α, PP2A-B56-δ, PP2A-B56-γ, PP2A-C 和 PP2A-C alpha / β

PP2A-Cα/β抗体（1D6）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人、牛、猪和犬的PP2A-Cα/β。抗PP2A-Cα/β抗体（1D6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。PP2A-Cα/β作为丝氨酸/苏氨酸磷酸酶在细胞信号传导中发挥着至关重要的作用，对于调节细胞分裂、分化、凋亡等多种细胞过程至关重要。PP2A-Cα/β主要位于细胞质和细胞核中，与各种底物相互作用，调节其磷酸化状态，从而影响关键的信号传导通路。PP2A-Cα/β的正常运作对于维持细胞内环境至关重要，失调与多种疾病有关，包括癌症和神经退行性疾病。PP2A家族由多个调节亚基组成，可与催化亚基结合，根据特定的细胞环境产生不同的功能结果。在研究中使用PP2A-Cα/β (1D6)抗体可以深入了解这种重要磷酸酶所控制的复杂调节机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PP2A-Cα/β 抗体 (1D6) 参考文献:

  1. 小鼠钙调蛋白依赖性蛋白磷酸酶调节亚基（钙调蛋白 B）两种异构体的结构和表达。  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
  2. 白血病中PP2A和microRNA的相互作用。  |  Ruvolo, PP. 2015. Front Oncol. 5: 43. PMID: 25750899
  3. 脑功能、发育和神经疾病中的蛋白磷酸酶 2A（PP2A）突变。  |  Verbinnen, I., et al. 2021. Biochem Soc Trans. 49: 1567-1588. PMID: 34241636
  4. 蛋白磷酸酶 2A (PP2A)的偏向全酶组装： 从癌症到小分子。  |  Haanen, TJ., et al. 2022. J Biol Chem. 298: 102656. PMID: 36328247
  5. PAK6介导的PPP2R2C磷酸化调节LRRK2-PP2A复合物的形成。  |  Iannotta, L., et al. 2023. Front Mol Neurosci. 16: 1269387. PMID: 38169846
  6. PP2A-B55磷酸酶在细胞分裂过程中抵消Ki-67依赖的染色体个体化。  |  Sanz-Flores, M., et al. 2024. Cell Rep. 43: 114494. PMID: 39003739
  7. 蛋白磷酸酶-1 的核抑制剂 NIPP-1 的分子克隆揭示了其与参与 RNA 处理的多肽的同源性。  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
  8. 丝氨酸/苏氨酸蛋白磷酸酶。  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
  9. 新型蛋白磷酸酶对丝氨酸和苏氨酸残基去磷酸化的重要作用  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
  10. 平滑肌肌球蛋白结合磷酸酶的调节亚基。  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
  11. 蛋白磷酸酶 2A 72 kDa 调节亚基的结构和表达。替代剪接产生不同大小形式的证据。  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
  12. 蛋白丝氨酸/苏氨酸磷酸酶：结构, 调节和在细胞生长中的功能。  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

订购信息

产品名称产品编号规格价格数量收藏夹

PP2A-Cα/β 抗体 (1D6)

sc-80665
100 µg/ml
$316.00

PP2A-Cα/β (1D6): m-IgGκ BP-HRP 套装

sc-521162
100 µg Ab, 40 µg BP
$354.00

PP2A-Cα/β (1D6): m-IgG2b BP-HRP 套装

sc-548867
100 µg Ab; 10 µg BP
$354.00

PP2A-Cα/β 抗体 (1D6) AC

sc-80665 AC
500 µg/ml, 25% agarose
$416.00

PP2A-Cα/β 抗体 (1D6) HRP

sc-80665 HRP
200 µg/ml
$316.00

PP2A-Cα/β 抗体 (1D6) FITC

sc-80665 FITC
200 µg/ml
$330.00

PP2A-Cα/β 抗体 (1D6) PE

sc-80665 PE
200 µg/ml
$343.00

PP2A-Cα/β 抗体 (1D6) Alexa Fluor® 488

sc-80665 AF488
200 µg/ml
$357.00

PP2A-Cα/β 抗体 (1D6) Alexa Fluor® 546

sc-80665 AF546
200 µg/ml
$357.00

PP2A-Cα/β 抗体 (1D6) Alexa Fluor® 594

sc-80665 AF594
200 µg/ml
$357.00

PP2A-Cα/β 抗体 (1D6) Alexa Fluor® 647

sc-80665 AF647
200 µg/ml
$357.00

PP2A-Cα/β 抗体 (1D6) Alexa Fluor® 680

sc-80665 AF680
200 µg/ml
$357.00

PP2A-Cα/β 抗体 (1D6) Alexa Fluor® 790

sc-80665 AF790
200 µg/ml
$357.00

Can you recommend a positive control to use with PP2A-Cα/β (1D6): sc-80665 monoclonal antibody for Western blot?

Asked by: jenni
We recommend U-698-M (sc-364799), NIH/3T3 (sc-2210) or 3T3-L1 (sc-2243) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of PP2A-Cα/β in these cell lines.
Answered by: Technical Support 7
Date published: 2017-01-17
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Rated 4 out of 5 by from Nice and single band (WB)This antibody well recognizes the human PP2A-C in MDA-MB-231 cells around 36 kDa. Ab incubation: 1:1000 in 5% milk in TBST for 2h at RT.
Date published: 2021-04-30
Rated 5 out of 5 by from this antibody works wellThis has worked very well in WB in HK2 cell lysates and mice kidney tissue
Date published: 2017-11-20
Rated 5 out of 5 by from tested in HCC cell linesThis antibody work excellent in western blotting. I could get clear and stable single stripe in two different hcc cell lines.
Date published: 2017-08-18
Rated 5 out of 5 by from Great performance in WBChecked with siPP2A-MEF cell samples, and first dilute to 1:1000, too much. Then further dilute to 1:5000, even 1:10000, still work for getting clear results.
Date published: 2017-08-17
Rated 5 out of 5 by from ExcellentThis antibody is very good for western blot and shows strong signal in HepG2 cells.I used at 1:1000 dilution.
Date published: 2017-07-14
Rated 5 out of 5 by from Great antibodyWe recently received a sample of PP2A-Cα/β (1D6) antibody and evaluated it's performance and specificity by Western Blot analysis using two different whole cell lysates from HEK 293 and 4 dpf zebrafish larvae (dpf, days post fertilization). There was a single band corresponding to PP2A-C (~36kDa) in both cases.
Date published: 2017-06-30
Rated 5 out of 5 by from Great antibody!I used this antibody for WB ,the protein was extracted from h9c2,the band is clear and special!
Date published: 2017-04-10
Rated 5 out of 5 by from screening my target protienI wanted to use this PP2A-C alpha / beta antibody to get my experiment more completed.
Date published: 2017-03-24
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PP2A-Cα/β 抗体 (1D6) is rated 4.9 out of 5 by 15.
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