PKC θ Pseudo-substrate inhibitor 抑制剂 MF: C91H157N35O21S
MW: 2109.5
Inhibitor for in vitro PKCθ kinase assays.

PKC θ Pseudo-substrate inhibitor 抑制剂

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备用名: Protein Kinase Cθ Pseudosubstrate Inhibitor; LHQRRGAIKQAKVHHVKC
应用; PKC θ Pseudo- substrate Inhibitor is inhibitor for in vitro PKCθ kinase assays
纯度: ≥97% by HPLC
分子量: 2109.5
分子式: C91H157N35O21S
仅供科研使用。不可用于诊断或治疗。
* 参考分析证明 大量特定数据 (包括水 含量).
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PKC θ Pseudo-substrate inhibitor is a protein kinase Cθ pseudosubstrate peptide that can be used as a competitive inhibitor for in vitro PKC θ kinase assays.


参考文献

1. Osada, S., Mizuno, K., Saido, T.C., Suzuki, K., Kuroki, T. and Ohno, S. 1992. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. Mol. Cell. Biol. 12(9): 3930-3938. PMID: 1508194

用法 :
Recommended reaction conditions: PKCθ-containing sample is incubated at 30 - 37°C, with or without 400 μM PKC θ Pseudo-substrate inhibitor, in 50 mM Tris buffer (pH 7.5) containing 5 mM MgCl2, 0.1 mM Na3VO4, 0.1 mM Na4P2O7, 1 mM NaF, 0.1 mM PMSF, 50 μM ATP, 5 μCi [γ-32P]ATP, 4 μg phosphatidylserine, and 40 μM PKCθ substrate (sc-3107).

Storage:Protect from moisture. After reconstitution, aliquot and freeze (-20°C). This product is stable for 2 years as supplied. Stock solutions are stable for 6 months -20°C.
外观 :
Lyophilized
物理状态 :
Solid
顺序 :
Leu-His-Gln-Arg-Arg-Gly-Ala-Ile-Lys-Gln-Ala-Lys-Val-His-His-Val-Lys-Cys-OH
溶解度 :
Soluble in water.
保存 :
Store at -20° C
仅供科研使用。不可用于诊断或治疗。

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PMID: # 28369981  Wang, Y.|Zhou, Q.|Wu, B.|Zhou, H.|Zhang, X.|Jiang, W.|Wang, L.|Wang, A.| et al. 2017. Br J Pharmacol. 174: 1984-2000.

PMID: # 25277212  Leung, CS. et al. 2014. Nature communications. 5: 5092.

PMID: # 19706599  Gayen, JR. et al. 2009. The Journal of biological chemistry. 284: 28498-509.

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