Phosphate Buffered Saline: 1 L of 1X Ready to use 1X PBS solution frequently used as a wash and dilution buffer in biological research applications.

Phosphate Buffered Saline: 1 L of 1X

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备用名: Phosphate Buffered Saline: 1 L of 1X is also known as 1X PBS.
应用; Phosphate Buffered Saline: 1 L of 1X is ready to use 1X PBS solution frequently used as a wash and dilution buffer in biological research applications.
仅供科研使用。不可用于诊断或治疗。
* 参考分析证明 大量特定数据 (包括水 含量).
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PBS, 1X liquid (Phosphate buffered saline) is a ready to use buffer solution commonly used in biological research. PBS buffer is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption. Additional sizes, formats, and concentrations available: PBS: 100 ml of 10X (sc-362297) PBS: 500 ml of 10X (sc-362298) PBS: 1 L of 10X (sc-24946) PBS: 1 L of 20X (sc-362183) PBS: 5 L of 20X (sc-362299) PBS: powder for 100 ml of 10X (sc-362300) PBS: powder for 500 ml of 10X (sc-362301) PBS: powder for 1 L of 10X (sc-362302) PBS: powder for 5 L of 10X (sc-24947)


参考文献

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792
2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548
3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994
4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324
5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070
6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.

用法 :
• Ready to use 1X PBS buffer is a solution with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. pH 7.4 ±0.2 (25°C).


• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
物理状态 :
Liquid
pH :
~7.4
保存 :
Store at room temperature
仅供科研使用。不可用于诊断或治疗。

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