Date published: 2025-9-8
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p-Atm Antibody (10H11.E12) - Western Blotting - Image 385740
p-Atm 抗体 (10H11. E12) HRP: sc-47739 HRP. 在 HeLa (A), HEL 92. 1. 7 (B), Raji (C) 和 Jurkat (D) 全细胞裂解液中 p-Atm 表达的直接 Western 印迹分析.
p-Atm 抗体 (10H11. E12) HRP: sc-47739 HRP. 在 HeLa (A), HEL 92. 1. 7 (B), Raji (C) 和 Jurkat (D) 全细胞裂解液中 p-Atm 表达的直接 Western 印迹分析.

p-Atm 抗体 (10H11.E12): sc-47739

4.3(20)
寫評論提問

说明书
  • p-Atm抗体(10H11.E12)是小鼠单克隆IgG1 κ, 在126篇文献中引用，规格为200 µg/ml
  • 与human来源的ATM的Ser 1981磷酸化位点周围的氨基酸Ser 1981对应的合成磷酸化肽的免疫反应
  • 抗-p-Atm 抗体 (10H11.E12) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Ser 1981 phosphorylated Atm
  • 抗p-Atm抗体（10H11.E12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Atm (10H11.E12): sc-47739的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 p-Atm 抗体 (10H11.E12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-Atm 抗体 (10H11.E12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p-Atm抗体（10H11.E12）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的磷酸化Atm Ser 1981。p- Atm (10H11.E12)抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Atm蛋白在细胞对DNA损伤的反应中起着至关重要的作用，特别是在检测和修复双链断裂方面。该功能可维持基因组的稳定性，因为Atm基因突变与共济失调毛细血管扩张症（AT）有关，会导致严重的神经和免疫缺陷。为了应对DNA损伤，Atm通过丝氨酸1981的自身磷酸化迅速激活，启动一系列信号传导事件，导致细胞周期停滞和DNA修复。大多数人体组织主要表达非磷酸化、非活性的Atm，这凸显了翻译后修饰在调节活性和功能方面的重要性。抗Atm抗体（10H11.E12）可特异性识别磷酸化Atm，因此p-Atm（10H11.E12）单克隆抗体成为研究DNA损伤反应机制和相关疾病病理生理学的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Atm 抗体 (10H11.E12) 参考文献:

  1. ATM信号和基因组稳定性对DNA损伤的响应  |  Lavin, MF., et al. 2005. Mutat Res. 569: 123-32. PMID: 15603757
  2. 共济失调-特朗根氏病突变激酶 ATM 的新型特异性阻抑剂的鉴定和特性分析。  |  Hickson, I., et al. 2004. Cancer Res. 64: 9152-9. PMID: 15604286
  3. 脓胸相关淋巴瘤中DNA损伤应答基因ATM和ATR的改变  |  Liu, A., et al. 2005. Lab Invest. 85: 436-46. PMID: 15696190
  4. 共济失调-特朗根氏症突变的阿特米斯在 DNA 损伤时的依赖性磷酸化。  |  Chen, L., et al. 2005. Cancer Sci. 96: 134-41. PMID: 15723659
  5. 正常人体组织和睾丸癌中的 ATM 激活。  |  Bartkova, J., et al. 2005. Cell Cycle. 4: 838-45. PMID: 15846060
  6. 磷酸化的共济失调-毛细血管扩张突变基因的表达减少与胰腺癌预后不良和吉西他滨耐药性有关。  |  Xun, J., et al. 2023. BMC Cancer. 23: 835. PMID: 37674118
  7. PIK 相关激酶：DNA 修复, 重组和细胞周期检查点。  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
  8. DNA 依赖性蛋白激酶催化亚基：磷脂酰肌醇 3-激酶和共济失调毛细血管扩张症基因产物的亲缘关系。  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
  9. AT基因的发现引发了生物医学研究的繁荣。  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
  10. 单个共济失调毛细血管扩张症基因的产物与 PI-3 激酶相似。  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

订购信息

产品名称产品编号规格价格数量收藏夹

p-Atm 抗体 (10H11.E12)

sc-47739
200 µg/ml
$316.00

p-Atm (10H11.E12): m-IgG Fc BP-HRP 套装

sc-528513
200 µg Ab; 10 µg BP
$354.00

p-Atm (10H11.E12): m-IgGκ BP-HRP 套装

sc-520908
200 µg Ab, 40 µg BP
$354.00

p-Atm (10H11.E12): m-IgG1 BP-HRP 套装

sc-542970
200 µg Ab; 20 µg BP
$354.00

p-Atm 抗体 (10H11.E12) AC

sc-47739 AC
500 µg/ml, 25% agarose
$416.00

p-Atm 抗体 (10H11.E12) HRP

sc-47739 HRP
200 µg/ml
$316.00

p-Atm 抗体 (10H11.E12) FITC

sc-47739 FITC
200 µg/ml
$330.00

p-Atm 抗体 (10H11.E12) PE

sc-47739 PE
200 µg/ml
$343.00

p-Atm 抗体 (10H11.E12) Alexa Fluor® 488

sc-47739 AF488
200 µg/ml
$357.00

p-Atm 抗体 (10H11.E12) Alexa Fluor® 546

sc-47739 AF546
200 µg/ml
$357.00

p-Atm 抗体 (10H11.E12) Alexa Fluor® 594

sc-47739 AF594
200 µg/ml
$357.00

p-Atm 抗体 (10H11.E12) Alexa Fluor® 647

sc-47739 AF647
200 µg/ml
$357.00

p-Atm 抗体 (10H11.E12) Alexa Fluor® 680

sc-47739 AF680
200 µg/ml
$357.00

p-Atm 抗体 (10H11.E12) Alexa Fluor® 790

sc-47739 AF790
200 µg/ml
$357.00

Dear Santa Cruz. We purchased pATM (sc-47739) and I cannot get the western blots to detect it. Could you please send me info on how to extract the protein (cells/heart tissue), and what WB steps you recommend to get it to work? Very much appreciated.

Asked by: Nick
Thank you for contacting Santa Cruz Biotechnology. Here is our protocol :https://www.scbt.com/resources/protocols/western-immuno-blotting. To trouble shoot the antibody please call 800-457-3801 or email scbt@scbt.com to speak with Technical Service.
Answered by: BlakeJ
Date published: 2025-05-29

Could you tell us incubation conditions of the antibody (blocking, buffer etc

Asked by: SPDeb
Thank you for contacting Santa Cruz Biotechnology. To better assist you please contact technical service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-02-06

Hi All, Are these antibodies p-ATM Antibody(10H11.E12):sc-47739(Alexa flour 647)and P53(sc-126 Alexa flour647) suitable to detect ATM and P53 in Bone marrow mouse samples using flow cytometry? Kind regards, Ahmad

Asked by: Ahmad Habib
Thank you for your question. The fluorescent conjugate versions of these antibodies can be used in flow cytometry.
Answered by: Tech Support Europe
Date published: 2023-08-11

What is a good negative control to test for phospho-specificity of this antibody?

Asked by: TinTin
Thank you for your question. We recommend treating your samples with lambda protein phosphatase (sc-200312A) as a control. Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins. As indicated in the Western blot data on our website and datasheet for this antibody (p-Atm Antibody (10H11.E12): sc-47739), treated samples should show a negative result when using this phospho-specific antibody in WB compared to the control phosphorylated sample.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 1 out of 5 by from AF-647 Does not work for IFNo signal even at high dilution (1/50) and use of a secondary Ab. I am used to work with this clone from another provider and I really got nice foci in nuclei after DNA damage
Date published: 2022-07-11
Rated 4 out of 5 by from good antibodygood antibody clean and strong signal in western blot or IF
Date published: 2021-11-15
Rated 4 out of 5 by from p ATM è un buon anticorpoL'anticorpo è stato testato su cellule U373 e ha dato buoni segnali diluito 1:1000
Date published: 2021-09-28
Rated 5 out of 5 by from 功能很好我很想试试这个抗体表达，很好用，实验必须用到，一直想买的抗体，这个有试用还挺好的，非常感谢，非常好的公司
Date published: 2021-05-23
Rated 5 out of 5 by from Very goodI tested this antibody in a context of DNA damage induction and it worked very well, giving a strong positive signal in human HeLa cells treated with bleomycin (using a dilution of 1:200 for imunocytochemistry)
Date published: 2021-02-10
Rated 4 out of 5 by from good antibodyIt worked nicely with my human cell lysates, better than I had from other company
Date published: 2018-01-29
Rated 4 out of 5 by from good antibodyThis antibody is good for immunofluorescence. (used at 1:100 dilution)
Date published: 2017-09-29
Rated 4 out of 5 by from Good antibody for pAtmI tested a sample antibody and it worked well in immunofluorescence.
Date published: 2017-09-24
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p-Atm 抗体 (10H11.E12) is rated 4.3 out of 5 by 20.
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