Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
NSUN5 Antibody (H-10) is a high quality monoclonal NSUN5 antibody (also designated NSUN5 antibody) suitable for the detection of the NSUN5 protein of human origin. NSUN5 Antibody (H-10) is available as both the non-conjugated anti-NSUN5 antibody form, as well as multiple conjugated forms of anti-NSUN5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. NSUN5 (NOL1/NOP2/Sun domain family, member 5), also known as NOL1, p120 or NOL1R, is a 429 amino acid protein that belongs to the methyltransferase superfamily and exists as multiple alternatively spliced isoforms. Expressed ubiquitously with higher expression in heart, placenta and skeletal muscle, NSUN5 is thought to function as an S-adenosyl-L-methionine-dependent methyl-transferase whose absence may be associated with the pathogenesis of Williams syndrome. NSUN5 is subject to post-translational phosphorylation, probably by ATM or ATR. The gene encoding NSUN5 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Defects in some of the genes localized to chromosome 7 have been linked to Osteogenesis imperfecta, Williams syndrome, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. We have not yet tested this antibody, NSUN5 (H-10): sc-376147, on formalin-fixed paraffin-embedded tissue sections and so the antibody is not recommended for IHC(P) at this time.
Answered by: Technical Support
Date published: 2017-11-02
What is the recommended fixation method for immunofluorescence staining with NSUN5 (H-10): sc-376147 monoclonal antibody?
Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: