Npl4 Double Nickase Plasmid (h): sc-403787-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Npl4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Npl4 Double Nickase Plasmid (h) and Npl4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NPLOC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Npl4 Antibody (D-1): sc-365796
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Npl4 Double Nickase Plasmid (h) | sc-403787-NIC | 20 µg | $410.00 | |||
Npl4 Double Nickase Plasmid (h2) | sc-403787-NIC-2 | 20 µg | $410.00 |
NPLOC4 encodes Npl4, a core cofactor of the p97/VCP AAA+ ATPase complex that recognizes polyubiquitinated substrates and couples ubiquitin-dependent extraction to proteasomal degradation. Through its role in ER-associated degradation (ERAD), ribosome-associated quality control, and chromatin-associated protein turnover, Npl4 helps maintain proteostasis during cellular stress and supports genome stability. Disruption of the p97–UFD1–Npl4 axis perturbs ubiquitin signaling and can alter responses to proteotoxic stress, DNA repair demands, and cell-cycle progression, making NPLOC4 a useful node for studying protein quality control networks. Aberrant regulation of this pathway has been linked to neurodegeneration- and cancer-relevant phenotypes in experimental systems via effects on protein homeostasis and stress-adaptive signaling.
Npl4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NPLOC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NPLOC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NPLOC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NPLOC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.