Mucin 5B抗体 (5B#19-2E) 推荐用于 mouse, rat 和 human 来源的Mucin 5B WB, IP 和 IF检测

Mucin 5B 抗体 (5B#19-2E): sc-21768

Mucin 5B抗体 (5B#19-2E) is rated 5.0 out of 5 by 7.
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说明书

    • 抗-Mucin 5B 抗体 5B#19-2E 是小鼠单克隆 IgG1 κ,Mucin 5B抗体, 在12篇文献中引用,规格为200 µg/ml
    • raised against a peptide mapping at the N-terminus of Mucin 5B of human origin
    • 抗-Mucin 5B 抗体 (5B#19-2E) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 Mucin 5B
    • Mucin 5B 抗体 (5B#19-2E) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Mucin 5B (5B#19-2E): sc-21768免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for Mucin 5B Antibody (5B#19-2E) for WB applications. These reagents are now offered in bundles with Mucin 5B Antibody (5B#19-2E) (see ordering information below).

What is the recommended fixation method for immunofluorescence staining with Mucin 5B (5B#19-2E): sc-21768 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IF on mouse lungs works great with this ab Immunofluorescence staining on mouse lungs works great with this ab.
Date published: 2018-09-19
Rated 5 out of 5 by from 一直在用一抗,因为停产,所以需要更换新的货号 一直在用一抗SC-20119,因为停产,所以需要更换新的货号Mucin 5B (5B#19-2E).
Date published: 2018-05-02
Rated 5 out of 5 by from Great staining A great antibody when in comes to IF and IHC. The signal is bright, clear, and specific. This antibody reacts well with mouse, rat, and human samples and has been used multiple times for publishing.
Date published: 2017-02-08
Rated 5 out of 5 by from Compliance statement Used to verify the mucin protein level in the cervical mucus and the peak at the central peak of the correlation, we purchased this product, product features consistent with the purchase instructions
Date published: 2017-01-18
Rated 5 out of 5 by from Worked for IF with methanol Worked for IF with methanol-fixed MCF7 cells. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Publication in IHC with human and mouse lung Publication in IHC with human and mouse lung samples. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Good WB data with human tracheobronchial Good WB data with human tracheobronchial epithelial culture and human saliva. -SCBT QC
Date published: 2013-08-20
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