Date published: 2025-9-7

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Mucin 5B/MUC5B 抗体 (5B#19-2E): sc-21768

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说明书
  • Mucin 5B/MUC5B 抗体 5B#19-2E 是小鼠单克隆 IgG1 κ,Mucin 5B/MUC5B抗体, 在19篇文献中引用,规格为200 µg/ml
  • 与氨基酸序列N-terminus(h)对应的多肽序列(Mucin 5B的human起源)
  • Mucin 5B/MUC5B 抗体 (5B#19-2E) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 Mucin 5B
  • Mucin 5B/MUC5B抗体(5B#19-2E)可与琼脂糖结合用于IP,与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mucin 5B/MUC5B (5B#19-2E): sc-21768免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Mucin 5B/MUC5B Antibody (5B#19-2E) for WB applications. 的首选辅助检测试剂,这些试剂现与Mucin 5B/MUC5B Antibody (5B#19-2E) 打包提供(请参阅下面的订购信息)。

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関連項目

Mucin 5B 抗体(5B#19-2E)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类来源的 Mucin 5B(MUC5B)。抗MUC5B抗体(5B#19-2E)有非结合型和多种结合型,包括琼脂糖、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合型。MUC5B是一种高分子量糖蛋白,在各种组织中形成保护性粘液层方面发挥着关键作用,特别是在呼吸和胃肠道中。MUC5B的功能是捕获病原体和微粒物质,作为抵御感染的第一道防线,并维持黏膜完整性。MUC5B主要在胆囊和结肠中表达,在慢性炎症反应中表达显著增加,特别是在中耳,疾病状态下分泌增加。MUC5B还与胃癌有关,其表达受远端启动子调节,远端启动子受蛋白激酶C活性和甲基化状态的影响。MUC5B的表达还受到视黄酸的进一步调节,而视黄酸对于粘液分化至关重要,这凸显了MUC5B在正常生理和疾病过程中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mucin 5B/MUC5B 抗体 (5B#19-2E) 参考文献:

  1. 宫颈粘液中 MUC5B 粘蛋白的含量在周期中期达到峰值。  |  Gipson, IK., et al. 2001. J Clin Endocrinol Metab. 86: 594-600. PMID: 11158014
  2. 气管支气管上皮粘液分化和粘蛋白基因表达的调控。  |  Gray, T., et al. 2001. Toxicology. 160: 35-46. PMID: 11246122
  3. 胃癌和癌细胞中人类粘蛋白基因 MUC5B 的异常表达。远端启动子的识别和调控。  |  Perrais, M., et al. 2001. J Biol Chem. 276: 15386-96. PMID: 11278696
  4. 促炎细胞因子肿瘤坏死因子-α和白细胞介素-6上调 HT29-MTX 细胞的粘蛋白分泌。  |  Smirnova, MG., et al. 2001. Eur Cytokine Netw. 12: 119-25. PMID: 11282555
  5. 人胆囊癌中 MUC1 和 MUC2 的表达:临床病理学研究及与预后的关系。  |  Kashiwagi, H., et al. 2001. Oncol Rep. 8: 485-9. PMID: 11295067
  6. 人类中耳和咽鼓管中粘蛋白的特征。  |  Lin, J., et al. 2001. Am J Physiol Lung Cell Mol Physiol. 280: L1157-67. PMID: 11350794
  7. 粘蛋白 5B 启动子多态性与特发性肺纤维化有关,但与系统性硬化症或肉样瘤病的肺纤维化发展无关。  |  Stock, CJ., et al. 2013. Thorax. 68: 436-41. PMID: 23321605
  8. DUSP28 将 Mucin 5B 和 Mucin 16 的调控与 AsPC-1 人胰腺癌细胞的迁移和存活联系起来。  |  Lee, J., et al. 2016. Tumour Biol. 37: 12193-12202. PMID: 27230679
  9. 口腔扁平苔藓患者唾液和血清中的粘蛋白 5B  |  Agha-Hosseini, F., et al. 2017. Sci Rep. 7: 12060. PMID: 28935947
  10. 携带 MUC5B Rs35705950 风险等位基因的 COVID-19 存活患者血浆中的粘蛋白 5B 水平较高。  |  García-Carmona, S., et al. 2022. Curr Issues Mol Biol. 44: 3283-3290. PMID: 35892712
  11. 从 HT-29 MTX 细胞中鉴定出一种 42 kDa 核因子(NF1-MUC5B),它能与人类粘蛋白基因 MUC5B 的 3' 区域结合。  |  Pigny, P., et al. 1996. Biochem Biophys Res Commun. 220: 186-91. PMID: 8602841
  12. MUC5B 是人类胆囊中的主要粘蛋白,在结肠上皮细胞中也有表达。  |  van Klinken, BJ., et al. 1998. Am J Physiol. 274: G871-8. PMID: 9612268

订购信息

产品名称产品编号规格价格数量收藏夹

Mucin 5B/MUC5B 抗体 (5B#19-2E)

sc-21768
200 µg/ml
$316.00

Mucin 5B/MUC5B (5B#19-2E): m-IgG Fc BP-HRP 套装

sc-526615
200 µg Ab; 10 µg BP
$354.00

Mucin 5B/MUC5B (5B#19-2E): m-IgG1 BP-HRP 套装

sc-531988
200 µg Ab; 20 µg BP
$354.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) AC

sc-21768 AC
500 µg/ml, 25% agarose
$416.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) FITC

sc-21768 FITC
200 µg/ml
$330.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) PE

sc-21768 PE
200 µg/ml
$343.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) Alexa Fluor® 488

sc-21768 AF488
200 µg/ml
$357.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) Alexa Fluor® 546

sc-21768 AF546
200 µg/ml
$357.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) Alexa Fluor® 594

sc-21768 AF594
200 µg/ml
$357.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) Alexa Fluor® 647

sc-21768 AF647
200 µg/ml
$357.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) Alexa Fluor® 680

sc-21768 AF680
200 µg/ml
$357.00

Mucin 5B/MUC5B 抗体 (5B#19-2E) Alexa Fluor® 790

sc-21768 AF790
200 µg/ml
$357.00

Would you predict this antibody to cross react with swine muc5B

Asked by: Path
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-07

What is the recommended fixation method for immunofluorescence staining with Mucin 5B (5B#19-2E): sc-21768 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good product!It works for our ELISA. Easy to follow instructions.
Date published: 2022-11-30
Rated 5 out of 5 by from IF on mouse lungs works great with this abImmunofluorescence staining on mouse lungs works great with this ab.
Date published: 2018-09-19
Rated 5 out of 5 by from 一直在用一抗,因为停产,所以需要更换新的货号一直在用一抗SC-20119,因为停产,所以需要更换新的货号Mucin 5B (5B#19-2E).
Date published: 2018-05-02
Rated 5 out of 5 by from Great stainingA great antibody when in comes to IF and IHC. The signal is bright, clear, and specific. This antibody reacts well with mouse, rat, and human samples and has been used multiple times for publishing.
Date published: 2017-02-08
Rated 5 out of 5 by from Compliance statementUsed to verify the mucin protein level in the cervical mucus and the peak at the central peak of the correlation, we purchased this product, product features consistent with the purchase instructions
Date published: 2017-01-18
Rated 5 out of 5 by from Worked for IF with methanolWorked for IF with methanol-fixed MCF7 cells. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Publication in IHC with human and mouse lungPublication in IHC with human and mouse lung samples. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Good WB data with human tracheobronchialGood WB data with human tracheobronchial epithelial culture and human saliva. -SCBT QC
Date published: 2013-08-20
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Mucin 5B/MUC5B 抗体 (5B#19-2E) is rated 5.0 out of 5 by 8.
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