Mcl-1 Antibody (RC31): sc-53971

Mcl-1 Antibody (RC31) is a mouse monoclonal IgG1 kappa light chain antibody, raised against amino acids 1-327 of human Mcl-1 protein, and effectively detects Mcl-1 of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a pivotal member of the Bcl-2 family of proteins that regulate apoptosis, the programmed cell death essential for development and tissue homeostasis. As an anti-apoptotic protein, Mcl-1 promotes cell survival by binding to pro-apoptotic factors like Bak and Bax, preventing mitochondrial outer membrane permeabilization and the release of cytochrome c. Mcl-1 undergoes various post-translational modifications that critically regulate function and stability. Phosphorylation of Mcl-1 by kinases such as glycogen synthase kinase 3 (GSK3) can mark Mcl-1 for ubiquitination and subsequent proteasomal degradation, thus modulating anti-apoptotic activity. Conversely, deubiquitination can stabilize Mcl-1, enhancing the ability to inhibit apoptosis. These post-translational modifications are significant because they allow for rapid cellular responses to stress signals by dynamically adjusting Mcl-1 levels, thereby influencing cell fate decisions between survival and death. Dysregulation of Mcl-1′s post-translational modifications can lead to overexpression, contributing to the development and progression of various cancers by enabling malignant cells to evade apoptosis. Understanding the regulation of Mcl-1 through these modifications is therefore crucial in cancer research and therapeutic development. Anti-Mcl-1 antibody (RC31) offers researchers a reliable tool to study the expression and post-translational regulation of Mcl-1 in various biological contexts.

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.