LPS A mitogen for bone marrow derived B lymphocytes.

LPS

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LPS is rated 5.0 out of 5 by 6.
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备用名: LPS is also known as Lipopolysaccharide.
应用; LPS is a highly immunogenic major component of the cell wall of gram negative bacteria that strongly activates immune cells bearing the CD14/TLR4/MD2 rec
纯度: purified by phenol extraction
仅供科研使用。不可用于诊断或治疗。
* 参考分析证明 大量特定数据 (包括水 含量).
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LPS (Lipopolysaccharide) is an endotoxin which exists as a critical glycolipid component of the outer cell wall of gram-negative bacteria. LPS is a known, potent initiator of the proinflammatory response and induces proinflammatory cytokines in several cell types. Many of the cell signals induced by gram-negative bacteria are attributed to LPS and has been observed to also activate TNF receptor signaling pathways. The major receptor that transduces the LPS signal has been recognized as a member of the Toll-like receptor family. At least 10 members of the family have been identified to date and TLR4 appears to be the main LPS receptor although coreceptors are most likely involved. Studies note that in HMEC (human microvascular endothelial cells) cell lines, LPS activates apoptosis pathways via both NF-κB and JNK through TRAF6. Also reported that NF-κB inhibition does not potentiate LPS-induced death in HMEC cells yet will sensitive these cell to TNF-induced death. Additional studies using HMEC cell lines report that following LPS stimulation, TRAF6 also transmits an important endothelial cell survival signal in a situation of complete NF-κB blockade which may indicate that TRAF6 signals an NF-κB–independent antiapoptotic pathway in endothelial cells exposed to LPS. LPS is also known as Lipopolysaccharides, Lipopolysaccharides from E. coli O111:B4, and Bacterial lipopolysaccharides.


参考文献

1. Zhang, F.X., et al. 1999. J. Biol. Chem. 274: 7611-7614. PMID: 10075645

2. Hull, C., et al. 2002. J. Immunol. 169: 2611-2618. PMID: 12193732

3. Raschi, E., et al. 2003. Blood. 101: 3495-3500. PMID: 12531807

4. Pollet, I., et al. 2003. Blood. 102: 1740-1742. PMID: 12714497

5. Wong, F., et al. 2004. Blood. 103: 4520-4526. PMID: 14996708

6. Wang, J., et al. 2008. J. Immunol. 180: 8011-8019. PMID: 18523264

用法 :
For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solution or cell culture medium.
Storage/Stability:
Solutions at 1 mg/ml in buffer or culture medium are stable for ~1 month at 2–8° C. Frozen aliquots can be stored up to 2 years. Repeated freeze/thaw cycles are not recommended. Solutions should be stored in silanized containers, since LPS can bind to plastics and certain types of glass (especially at concentrations of <0.1 mg/ml). If the LPS concentration is >1 mg/ml, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve the adsorbed product.
外观 :
Lyophilized powder
物理状态 :
Solid
来源于 :
E. coli O111:B4
溶解度 :
Soluble in water (5 mg/ml), and cell culture medium (1 mg/ml).
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
3
PubChem CID :
MDL 号码 :
MFCD00164401
SMILES :
CCCCCCCCCCCCCC(=O)O[[email protected]](CCCCCCCCCCC)CC(=O)O[[email protected]@H]1[[email protected]]([[email protected]@H](O[[email protected]@H]([[email protected]]1OP(=O)(O)O)CO[[email protected]@]2(C[[email protected]]([[email protected]@H]([[email protected]](O2)[[email protected]](CO)O)OC3[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O3)[[email protected]](CO)O)OP(=O)(O)OP(=O)(O)OCCN)OC4[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O4)[[email protected]](COC5[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O5)[[email protected]](CO)O)OP(=O)(O)O)O)O)O)O)OC6[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O6)CO[[email protected]@H]7[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O7)CO)O)O)O)O)O[[email protected]@H]8[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O8)CO)O)O)OC9[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O9)CO)O)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O)O)NC(=O)C)O)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO)O)O[[email protected]@H]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO[[email protected]@H]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO)O)O)O)O)O)O)NC(=O)C)NC(=O)C)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O)O)NC(=O)C)O)O)O)O[[email protected]@]1(C[[email protected]]([[email protected]@H]([[email protected]](O1)[[email protected]](CO)O)O)O[[email protected]@]1(C[[email protected]]([[email protected]@H]([[email protected]](O1)[[email protected]](CO)O)O)OP(=O)(O)OCCN)C(=O)O)C(=O)O)C(=O)O)OC[[email protected]@H]1[[email protected]]([[email protected]@H]([[email protected]]([[email protected]](O1)OP(=O)(O)O)NC(=O)C[[email protected]@H](CCCCCCCCCCC)O)OC(=O)C[[email protected]@H](CCCCCCCCCCC)O)O)NC(=O)C[[email protected]@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC

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PMID: # 34192597  Pazoki, H.|Mohammad Rahimi, H.|Mirjalali, H.|Niyyati, M.|Mosaffa, N.|Seyed Tabaei, SJ.|Shahrokh, S.|Asadzadeh Aghdaei, H.|Zali, MR.| et al. 2021. Microb Pathog. 158: 105072.

PMID: # 32135404  Xu, X.|Hu, P.|Ma, Y.|Tong, L.|Wang, D.|Wu, Y.|Chen, Z.|Huang, C.| et al. 2020. J Nutr Biochem. 78: 108323.

PMID: # 30938885  Yang, J.|Li, R.|Zhao, D.|Zheng, S.| et al. 2019. J. Cell. Biochem. 120: 12887-12903.

PMID: # 30914954  Rossaneis, AC.|Longhi-Balbinot, DT.|Bertozzi, MM.|Fattori, V.|Segato-Vendrameto, CZ.|Badaro-Garcia, S.|Zaninelli, TH.|Staurengo-Ferrari, L.|Borghi, SM.|Carvalho, TT.|Bussmann, AJC.|Gouveia, FS.|Lopes, LGF.|Casagrande, R.|Verri, WA.| et al. 2019. Front Pharmacol. 10: 229.

PMID: # 29581852  Wang, Q.|Yang, X.|Xu, Y.|Shen, Z.|Cheng, H.|Cheng, F.|Liu, X.|Wang, R.| et al. 2018. Oncotarget. 9: 14397-14412.

PMID: # 29058362  Huang, C. et al. 2018. Glia. 66: 256-278.

PMID: # 30247686  Miklavcic, JJ.|Badger, TM.|Bowlin, AK.|Matazel, KS.|Cleves, MA.|LeRoith, T.|Saraf, MK.|Chintapalli, SV.|Piccolo, BD.|Shankar, K.|Yeruva, L.| et al. 2018. J. Nutr. 148: 1860-1870.

PMID: # 29844724  Al-Saeedan, AS.|Gautam, V.|Ansari, MN.|Singh, M.|Yadav, RK.|Rawat, JK.|Devi, U.|Gautam, S.|Roy, S.|Kaithwas, G.| et al. 2018. Saudi Pharm J. 26: 520-527.

PMID: # 28892097  Muku, GE.|Lahoti, TS.|Murray, IA.|Podolsky, MA.|Smith, KJ.|Hubbard, TD.|Kuzu, G.|Gowda, K.|Amin, SG.|Perdew, GH.| et al. 2017. Lab Invest. 97: 1471-1487.

PMID: # 28515460  Giakoumelou, S.|Wheelhouse, N.|Brown, J.|Wade, J.|Simitsidellis, I.|Gibson, D.|Saunders, PTK.|Horner, P.|Entrican, G.|Howie, SEM.|Horne, AW.| et al. 2017. Sci Rep. 7: 2001.

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