LI-cadherin CRISPR/Cas9 KO Plasmid (m): sc-419592
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LI-cadherin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LI-cadherin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LI-cadherin Antibody (H-1): sc-393533
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LI-cadherin CRISPR/Cas9 KO Plasmid (m) | sc-419592 | 20 µg | $397.00 |
Overview
Cdh17 encodes LI-cadherin, a calcium-dependent cell–cell adhesion molecule of the cadherin superfamily that contributes to epithelial cohesion and polarity in gastrointestinal and hepatobiliary tissues. Through homophilic adhesion at the cell surface, LI-cadherin helps organize junctional architecture, regulates barrier integrity, and influences contact-dependent control of cell migration and differentiation. Altered cadherin-mediated adhesion is linked to epithelial remodeling, dysplasia, and invasion-associated phenotypes, making Cdh17 a useful entry point for studying junctional stability during inflammation and tumor-associated progression in the mouse. Its expression pattern and adhesion function also support its use as a marker-connected target in studies of epithelial lineage identity and tissue organization.
LI-cadherin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cdh17 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cdh17 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cdh17 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LI-cadherin protein expression.
This CRISPR knockout system enables efficient generation of Cdh17-deficient cell models for investigation of LI-cadherin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cdh17 exon(s) critical for LI-cadherin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cdh17 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LI-cadherin CRISPR/Cas9 KO Plasmid (m) and LI-cadherin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cdh17 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LI-cadherin HDR Plasmid (m) and LI-cadherin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cdh17 homology arms to support homology-directed repair at defined Cdh17 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.