Date published: 2025-9-8

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karyopherin α2 抗体 (2G7): sc-57009

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说明书
  • karyopherin alpha 2 抗体 2G7 是大鼠单克隆 IgG2a,提供 200 µg/ml
  • 免疫human物种的karyopherin α2的氨基酸116-515
  • 推荐用于 mouse, rat 和 human 来源的karyopherin α2 WB 和 IP检测
  • 看 karyopherin α2 (B-9): sc-55538,可查看有标记的 karyopherin α2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成karyopherin α2 抗体 (2G7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    karyopherin α2 抗体(2G7)是一种大鼠单克隆 IgG2a 抗体,可通过免疫印迹(WB)和免疫沉淀(IP)等应用检测小鼠、大鼠和人样本中的 karyopherin α2。Karyopherin α2通过识别和结合特定底物在核导入过程中发挥着关键作用,这对基因表达和细胞功能的调控至关重要。Karyopherin α2主要位于细胞质中,抗 Karyopherin α2抗体(2G7)能检测到它与货物蛋白上的各种核定位信号的相互作用,从而促进它们穿过核膜的运输。Karyopherin α2参与病毒蛋白(如爱泼斯坦-巴氏病毒核抗原1(EBNA1))的导入,从而影响病毒的复制和致病机理,这凸显了Karyopherin α2功能的重要性。Karyopherin α2 (2G7) 抗体能让研究人员深入了解核转运的机制以及失调在癌症和病毒感染等各种疾病中的影响。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    karyopherin α2 抗体 (2G7) 参考文献:

    1. 核仁蛋白α2:肝细胞中葡萄糖敏感基因表达的控制步骤。  |  Guillemain, G., et al. 2002. Biochem J. 364: 201-9. PMID: 11988093
    2. 核仁蛋白α2核转运途径在肝细胞和胰腺细胞中受葡萄糖调节。  |  Cassany, A., et al. 2004. Traffic. 5: 10-9. PMID: 14675421
    3. 核仁蛋白α2对于增殖性角质形成细胞中的rRNA转录和蛋白质合成至关重要。  |  Umegaki-Arao, N., et al. 2013. PLoS One. 8: e76416. PMID: 24098495
    4. 沉默 karyopherin α2 可抑制人肝细胞癌细胞的生长和存活。  |  Yang, Y., et al. 2017. Oncotarget. 8: 36289-36304. PMID: 28422734
    5. 通过干扰 BRCA1 介导的 DNA 修复,抑制 Karyopherin-α2 可增强辐射诱导的细胞死亡。  |  Song, KH., et al. 2019. Int J Mol Sci. 20: PMID: 31212646
    6. 依赖核仁蛋白α2的E2F1和TFDP1导入维持肝癌中促肿瘤性stathmin的表达。  |  Drucker, E., et al. 2019. Cell Commun Signal. 17: 159. PMID: 31783876
    7. 核仁蛋白α2(KPNA2)在人类肿瘤中的致癌作用:一项泛癌分析。  |  Sun, Y., et al. 2021. Comput Biol Med. 139: 104955. PMID: 34735944
    8. 小细胞宫颈癌中核仁蛋白α2的过度表达与不良预后相关。  |  Liao, LM., et al. 2022. Int J Clin Exp Pathol. 15: 241-246. PMID: 35795090
    9. 威非特灵A通过上调靶向核仁蛋白α2的microRNA-1297,减轻关节炎大鼠的炎症和关节损伤。  |  Sheng, JD., et al. 2023. J Physiol Pharmacol. 74: PMID: 38345447
    10. 核仁蛋白α2是一种母体效应基因,对于小鼠早期胚胎发育和女性生育能力至关重要。  |  Rother, F., et al. 2024. FASEB J. 38: e23623. PMID: 38656660

    订购信息

    产品名称产品编号规格价格数量收藏夹

    karyopherin α2 抗体 (2G7)

    sc-57009
    200 µg/ml
    $316.00

    How much sample do you recommend loading on the gel for Western blot detection using karyopherin α2 (2G7): sc-57009?

    Asked by: jerojero
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from WB: robust band in BJAB whole cell lysateWB: robust band in BJAB whole cell lysate. -SCBT QC
    Date published: 2015-06-17
    Rated 5 out of 5 by from Positive results in HeLa whole cell lysatePositive results in HeLa whole cell lysate with western blotting. -SCBT QC
    Date published: 2015-02-22
    Rated 4 out of 5 by from Good specificity and signal strength withGood specificity and signal strength with some background bands in western blot with Jurkat and transfected 293T whole cell lysates. -SCBT QC
    Date published: 2015-02-01
    Rated 5 out of 5 by from Strong bands in western blot analysis ofStrong bands in western blot analysis of karyopherin 2 expression in HeLa and MCF7 nuclear extracts. -SCBT QC
    Date published: 2014-02-11
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    karyopherin α2 抗体 (2G7) is rated 4.8 out of 5 by 4.
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