Date published: 2025-9-6

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Importin-7 抗体 (E-2): sc-365231

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说明书
  • Importin-7抗体(E-2)是小鼠单克隆IgG2b λ Importin-7抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列961-1038位于human物种的Importin-7的C-terminus
  • Importin-7 抗体 (E-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Importin-7
  • 抗Importin-7抗体(E-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Importin-7 (E-2): sc-365231免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP(小鼠 IgGλ 结合蛋白-HRP)是Importin-7 Antibody (E-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与Importin-7 Antibody (E-2) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGλ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Importin-7抗体(E-2)是一种IgG2b λ小鼠单克隆Importin-7抗体(也称为IPO7抗体或RANBP7抗体),可通过WB, IP, IF, IHC(P)和ELISA检测小鼠、大鼠和人类的Importin-7蛋白。Importin-7抗体(E-2)既可作为非偶联的抗Importin-7抗体形式提供,也可作为多种偶联形式的抗Importin-7抗体提供,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。Importin-7(Ran结合蛋白7,RanBP7)是一种由人类基因IPO7编码的含有1038个氨基酸的蛋白质。Importin-7属于importin b家族,并含有一个importin N端结构域。Importin-7通过充当自主核转运受体或与Importin b亚基KPNB1相关的类似适配器的蛋白质,在核蛋白导入中发挥作用。Importin-7自主发挥作用时,被认为可以作为核定位信号(NLS)的受体,并通过需要能量且依赖于Ran的机制促进导入底物通过核孔复合物(NPC)的易位。在NPC的核质侧,Ran与Importin-7结合,Importin-7/底物复合物解离,Importin-7从细胞核重新输出到细胞质,在那里GTP水解释放Ran。Importin-7是一种在大多数组织中表达的核蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Importin-7 抗体 (E-2) 参考文献:

  1. 导入素β/导入素7异二聚体是组蛋白H1的功能性核导入受体。  |  Jäkel, S., et al. 1999. EMBO J. 18: 2411-23. PMID: 10228156
  2. 整合素与 moleskin(一种编码果蝇 importin-7 同源物的基因)之间的遗传相互作用。  |  Baker, SE., et al. 2002. Genetics. 162: 285-96. PMID: 12242240
  3. HIV-1 细胞内逆转录复合物的核导入是由导入素 7 介导的。  |  Fassati, A., et al. 2003. EMBO J. 22: 3675-85. PMID: 12853482
  4. 导入素 7 和导入素 alpha/importin beta 是糖皮质激素受体的核导入受体。  |  Freedman, ND. and Yamamoto, KR. 2004. Mol Biol Cell. 15: 2276-86. PMID: 15004228
  5. 人体免疫缺陷病毒 1 型和猿猴免疫缺陷病毒感染初级巨噬细胞时,导入素 7 可能是不可或缺的。  |  Zielske, SP. and Stevenson, M. 2005. J Virol. 79: 11541-6. PMID: 16103209
  6. 平滑蛋白和厚维素调节果蝇眼睛发育过程中由 Moleskin/Importin 7 介导的 MAP 激酶信号转导。  |  Vrailas, AD., et al. 2006. Development. 133: 1485-94. PMID: 16540506
  7. 多种导入蛋白作为人类免疫缺陷病毒 1 型 Rev 蛋白的核转运受体发挥作用。  |  Arnold, M., et al. 2006. J Biol Chem. 281: 20883-20890. PMID: 16704975

订购信息

产品名称产品编号规格价格数量收藏夹

Importin-7 抗体 (E-2)

sc-365231
200 µg/ml
$316.00

Importin-7 (E-2): m-IgGλ BP-HRP 套装

sc-522170
200 µg Ab, 40 µg BP
$354.00

Importin-7 抗体 (E-2) AC

sc-365231 AC
500 µg/ml, 25% agarose
$416.00

Importin-7 抗体 (E-2) HRP

sc-365231 HRP
200 µg/ml
$316.00

Importin-7 抗体 (E-2) FITC

sc-365231 FITC
200 µg/ml
$330.00

Importin-7 抗体 (E-2) PE

sc-365231 PE
200 µg/ml
$343.00

Importin-7 抗体 (E-2) Alexa Fluor® 488

sc-365231 AF488
200 µg/ml
$357.00

Importin-7 抗体 (E-2) Alexa Fluor® 546

sc-365231 AF546
200 µg/ml
$357.00

Importin-7 抗体 (E-2) Alexa Fluor® 594

sc-365231 AF594
200 µg/ml
$357.00

Importin-7 抗体 (E-2) Alexa Fluor® 647

sc-365231 AF647
200 µg/ml
$357.00

Importin-7 抗体 (E-2) Alexa Fluor® 680

sc-365231 AF680
200 µg/ml
$357.00

Importin-7 抗体 (E-2) Alexa Fluor® 790

sc-365231 AF790
200 µg/ml
$357.00

I am using Importin-7 (E-2): sc-365231 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Very works with western blottingThis antibody very works with western blotting with SAS cell lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from Very good antibodyWorks very nicely for Western blots (used at 1:500) and IF staining (used at 1:250). I tested the antibody on HeLa cells.
Date published: 2018-03-15
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from Good antibody.Produced excellent immunofluorescence nuclear membrane staining in PFA fixed RCTE cells. Recommend dilution is 1:100.
Date published: 2017-06-22
Rated 5 out of 5 by from Great for WesternsUsed for Western blotting WCE/Soluble/Chromatin fractions. Served as a great positive control to make sure my chromatin fraction was free of cytoplasmic/nucleoplasmic contaminants. Very strong, specific signal (used at 1 ug/mL).
Date published: 2017-03-17
Rated 5 out of 5 by from WB publishable data with human PASMCsWB publishable data with human PASMCs - SCBT Publication Review
Date published: 2015-01-27
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear stainingBeautiful immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2014-10-31
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Importin-7 抗体 (E-2) is rated 5.0 out of 5 by 7.
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