HPV16 E1/E4 Antibody (TVG 402): sc-53324

HPV16 E1/E4 Antibody (TVG 402) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HPV16 E1/E4 protein of HPV origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). HPV16 E1/E4 (TVG 402) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The human papillomavirus (HPV) family includes HPV16, a high-risk sexually transmitted virus associated with several cancers, including cervical, anal, vulvar, head, neck, and penile cancers. HPV16 E1/E4 protein plays a crucial role in modulating the cell cycle by inducing G2 phase arrest, which is essential for viral replication. HPV16 E1/E4 achieves this function by interacting with cyclin-dependent kinase 1 (Cdk1) and cyclin B1 complexes, preventing their nuclear entry and thereby inhibiting mitotic progression. Additionally, HPV16 E1/E4 interacts with cyclin A and Cdk2 during the G2 phase, enhancing its ability to block cell division. Notably, HPV16 E1/E4 also associates with keratin intermediate filaments, leading to the disruption of the keratin network, which is significant for the viral life cycle and pathogenesis. The ability of HPV16 E1/E4 to manipulate cellular processes underscores its importance in HPV-related oncogenesis and makes anti-HPV16 E1/E4 antibody (TVG 402) a valuable tool for research into HPV biology and its associated diseases.

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HPV16 E1/E4 Antibody (TVG 402) References:

1. The human papillomavirus type 11 E1E4 protein is phosphorylated in genital epithelium.  |  Bryan, JT., et al. 2000. Virology. 268: 430-9. PMID: 10704351
2. The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.  |  Doorbar, J., et al. 2000. J Virol. 74: 10081-95. PMID: 11024137
3. Identification of a G(2) arrest domain in the E1 wedge E4 protein of human papillomavirus type 16.  |  Davy, CE., et al. 2002. J Virol. 76: 9806-18. PMID: 12208959
4. Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.  |  Doorbar, J., et al. 1992. Virology. 187: 353-9. PMID: 1371027
5. Functional analysis of the human papillomavirus type 16 E1=E4 protein provides a mechanism for in vivo and in vitro keratin filament reorganization.  |  Wang, Q., et al. 2004. J Virol. 78: 821-33. PMID: 14694114
6. Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes.  |  Davy, CE., et al. 2005. J Virol. 79: 3998-4011. PMID: 15767402
7. HPV16 E1--E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm.  |  Davy, CE., et al. 2006. Virology. 349: 230-44. PMID: 16540140
8. Elevated HPV16 E1 Expression Is Associated with Cervical Cancer Progression.  |  Baedyananda, F., et al. 2017. Intervirology. 60: 171-180. PMID: 29495005
9. HnRNP D activates production of HPV16 E1 and E6 mRNAs by promoting intron retention.  |  Cui, X., et al. 2022. Nucleic Acids Res. 50: 2782-2806. PMID: 35234917
10. Role of HPV16 E1 in cervical carcinogenesis.  |  Baedyananda, F., et al. 2022. Front Cell Infect Microbiol. 12: 955847. PMID: 35967849
11. Identification of HPV16 E1 and E2-specific T cells in the oropharyngeal cancer tumor microenvironment.  |  McInnis, C., et al. 2023. J Immunother Cancer. 11: PMID: 36990508
12. The combined HPV16-E2/E6/E7 T cell response in oropharyngeal cancer predicts superior survival.  |  Santegoets, SJ., et al. 2023. Cell Rep Med. 4: 101262. PMID: 37924817

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV16 E1/E4 Antibody (TVG 402) has 4 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV16 E1/E4 Antibody (TVG 402) Specifications

Alternate Product Name: HPV16 E1/E4

Product Clone Name: TVG 402

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 36-41

Epitope Target: HPV16 E1/E4

Epitope Species: HPV

Product Reactivity/Detection: HPV16 E1 and HPV16 E4

Species Reactivity/Detection: HPV

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for HPV16 E1/E4 Antibody (TVG 402): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin